Anti-proliferative activity of Pylaiella littoralis extract on HT29cells through Caspase and MAPKs pathway

Title
Anti-proliferative activity of Pylaiella littoralis extract on HT29cells through Caspase and MAPKs pathway
Author(s)
예보람; 장지이; 김준성; 김민선; 김지형; 이영득; 이수진; 오철홍; 강도형; 허수진
KIOST Author(s)
Kim, Junseong(김준성)Lee, Youngdeuk(이영득)Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)Heo, Soo Jin(허수진)
Publication Year
2013-07-09
Abstract
This study demonstrated that Pylaiella littoralis extract (PLE) could inhibit the proliferation of tumor cells. PLE showed anti-proliferative activities in the tested tumorgenic cells ranged from 20.2% to 67.9%. The highest inhibitory activity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis.ity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26870
Bibliographic Citation
KSABC, pp.207, 2013
Publisher
한국응용생명화학회
Type
Conference
Language
English
Publisher
한국응용생명화학회
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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