Anti-proliferative activity of Pylaiella littoralis extract on HT29cells through Caspase and MAPKs pathway

Abstract

This study demonstrated that Pylaiella littoralis extract (PLE) could inhibit the proliferation of tumor cells. PLE showed anti-proliferative activities in the tested tumorgenic cells ranged from 20.2% to 67.9%. The highest inhibitory activity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis.ity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis.