Anti-proliferative activity of Pylaiella littoralis extract on HT29cells through Caspase and MAPKs pathway

DC Field Value Language
dc.contributor.author 예보람 -
dc.contributor.author 장지이 -
dc.contributor.author 김준성 -
dc.contributor.author 김민선 -
dc.contributor.author 김지형 -
dc.contributor.author 이영득 -
dc.contributor.author 이수진 -
dc.contributor.author 오철홍 -
dc.contributor.author 강도형 -
dc.contributor.author 허수진 -
dc.date.accessioned 2020-07-16T08:31:24Z -
dc.date.available 2020-07-16T08:31:24Z -
dc.date.created 2020-02-11 -
dc.date.issued 2013-07-09 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/26870 -
dc.description.abstract This study demonstrated that Pylaiella littoralis extract (PLE) could inhibit the proliferation of tumor cells. PLE showed anti-proliferative activities in the tested tumorgenic cells ranged from 20.2% to 67.9%. The highest inhibitory activity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis.ity of HT-29 cells was selected for use in further experiments. PLE exhibited no cytotoxic effect in normal cells and inhibited cell growth in HT-29 cells depending on concentration and time. We confirmed indicators of apoptosis such as nuclear condensation, apoptotic body formation, DNA fragmentation and sub-G1 DNA accumulation in HT-29 cells with treated PLE. PLE induced mitochondria membrane potential depolarization and resulted increase of mitochondrial membrane permeabilization compare with untreated PLE. PLE exhibited decreasing Bcl-2 protein, increasing Bax protein, activating Caspase-3 and PARP expressions via caspases pathway. Also, PLE increased expression of phosphorylation of JNK, P38 and ERK and attenuated specific inhibitors of JNK, P38 and ERK via MAPKs pathway. These results suggest that P. littoralis extract could inhibit the proliferation of HT-29 cells by Caspase and MAPK pathways involving the induction of apoptosis. -
dc.description.uri 1 -
dc.language English -
dc.publisher 한국응용생명화학회 -
dc.relation.isPartOf KSABC -
dc.title Anti-proliferative activity of Pylaiella littoralis extract on HT29cells through Caspase and MAPKs pathway -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 207 -
dc.citation.startPage 207 -
dc.citation.title KSABC -
dc.contributor.alternativeName 예보람 -
dc.contributor.alternativeName 장지이 -
dc.contributor.alternativeName 김준성 -
dc.contributor.alternativeName 김민선 -
dc.contributor.alternativeName 김지형 -
dc.contributor.alternativeName 이영득 -
dc.contributor.alternativeName 이수진 -
dc.contributor.alternativeName 오철홍 -
dc.contributor.alternativeName 강도형 -
dc.contributor.alternativeName 허수진 -
dc.identifier.bibliographicCitation KSABC, pp.207 -
dc.description.journalClass 1 -
Appears in Collections:
Jeju Research Institute > Jeju Marine Research Center > 2. Conference Papers
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