A novel Archael type CO sensing regulator: RchA

Title
A novel Archael type CO sensing regulator: RchA
Author(s)
김민식; 강성균; 최애란; 이성혁; 이정현; 이현숙
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)
Publication Year
2013-09-13
Abstract
Carbon monoxide (CO) is a notorious toxic gas that can cause sometimes suffocation by poisoning. This toxicity comes from its strong affinity to metals. Due to its strong affinity to haemoglobin, it can block tissue oxygenation and also inhibit mitochondrial electron transport chain by binding cytochrome. On the other hand, some microorganisms are able to get benefit from CO. CO is not only a key factor for autotrophic pathway such as methanogenesis and acetogenesis but an energy source for carboxydotrophic bacteria like Carboxydothermus hydrogenoformans and Rhodospirillium rubrum. The carboxydotrophic bacteria encode energy conserving hydrogenase gene cluster (mch) linked to carbon monoxide dehydrogenase (CODH) in their genome and CooA type CO sensing regulator is responsible for timely expression of this cluster in response to CO. CooA can sense the presence of CO through heme, and CO binding to heme causes conformational change that leads to transcriptional activation of CODH-mch cluster. RcoM is another CO-sensing regulator from Burkholderia xenovorans and similar to CooA in that it takes advantage of heme for CO binding. Thermococcus onnurineus NA1 was reported to harbor CODH-mch cluster essential for CO-dependent growth. Transcriptional and translational regulation of this cluster in response to CO was confirmed by RT-qPCR and western blotting but there is no CO sensing regulator in the genome homologous to Cibit mitochondrial electron transport chain by binding cytochrome. On the other hand, some microorganisms are able to get benefit from CO. CO is not only a key factor for autotrophic pathway such as methanogenesis and acetogenesis but an energy source for carboxydotrophic bacteria like Carboxydothermus hydrogenoformans and Rhodospirillium rubrum. The carboxydotrophic bacteria encode energy conserving hydrogenase gene cluster (mch) linked to carbon monoxide dehydrogenase (CODH) in their genome and CooA type CO sensing regulator is responsible for timely expression of this cluster in response to CO. CooA can sense the presence of CO through heme, and CO binding to heme causes conformational change that leads to transcriptional activation of CODH-mch cluster. RcoM is another CO-sensing regulator from Burkholderia xenovorans and similar to CooA in that it takes advantage of heme for CO binding. Thermococcus onnurineus NA1 was reported to harbor CODH-mch cluster essential for CO-dependent growth. Transcriptional and translational regulation of this cluster in response to CO was confirmed by RT-qPCR and western blotting but there is no CO sensing regulator in the genome homologous to C
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26788
Bibliographic Citation
12th International Thermophiles Conference, pp.64, 2013
Publisher
12th International meeting Thermophiles
Type
Conference
Language
English
Publisher
12th International meeting Thermophiles
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse