Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum
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Title
- Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum
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Author(s)
- Cho, MyoungLae; Lee, Dong-Jin; Kim, Jin-Kyung; You, SangGuan
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Alternative Author(s)
- 조명래
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Publication Year
- 2014-11-26
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Abstract
- The sulfated-fucans, known as fucoidans, were isolated from Agarum cribrosum and fractionated using ion-exchange chromatography to determine their molecular characteristics and in vitro immunomodulatory activity. The crude and fractionated fucoidans (F-1 and F-2) consisted mostly of carbohydrates (52.4-56.0%), sulfates (12.7-23.0%) and uronic acid (14.1-21.8%), with a small amount of proteins (3.9-9.3%), and included various levels of fucose (44.0-46.7%), mannose (18.9-26.8%), galactose (16.8-33.0%), xylose (10.7-17.0%) and glucose (3.5-9.5%). The crude and fractionated fucans contained one or two subfractions with average molecular weights (M-w) ranging from 110.1 x 10(3) to 2420 x 10(3) g/mol. The fractionated fucoidan, especially the F-1 fraction, strongly stimulated murine macrophages (Raw 264.7 cells), producing a considerable amount of nitric oxide (NO) and inducing expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and interleukin-10 (IL-10) transcripts by activation of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinases (MAPKs) pathways. The maximally immunoenhancing F1 fraction was mainly composed of (1 -> 3)-linked fucose, (1 -> 2)-linked mannose and (1 -> 4)-linked glucuronic acid with sulfates at C-2 or both the C-2 and C-4 positions in (1 -> 2,3)- and (1 -> 2,3,4)-linked fucose residues. (C) 2014 Elsevier Ltd. All rights reserved.
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ISSN
- 0144-8617
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2673
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DOI
- 10.1016/j.carbpol.2014.07.055
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Bibliographic Citation
- CARBOHYDRATE POLYMERS, v.113, pp.507 - 514, 2014
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Publisher
- ELSEVIER SCI LTD
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Subject
- NF-KAPPA-B; UNDARIA-PINNATIFIDA; STRUCTURAL-ANALYSIS; ECKLONIA-CAVA; IN-VITRO; FUCOIDAN; POLYSACCHARIDES; CELLS; SPOROPHYLL; FRACTIONS
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Keywords
- Agarum cribrosum; Sulfated-fucans; Immunomodulation; Glycosidic linkage; NF-kappa B; MAPK
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Type
- Article
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Language
- English
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Document Type
- Article
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