Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Cho, MyoungLae | - |
dc.contributor.author | Lee, Dong-Jin | - |
dc.contributor.author | Kim, Jin-Kyung | - |
dc.contributor.author | You, SangGuan | - |
dc.date.accessioned | 2020-04-20T04:25:19Z | - |
dc.date.available | 2020-04-20T04:25:19Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2014-11-26 | - |
dc.identifier.issn | 0144-8617 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2673 | - |
dc.description.abstract | The sulfated-fucans, known as fucoidans, were isolated from Agarum cribrosum and fractionated using ion-exchange chromatography to determine their molecular characteristics and in vitro immunomodulatory activity. The crude and fractionated fucoidans (F-1 and F-2) consisted mostly of carbohydrates (52.4-56.0%), sulfates (12.7-23.0%) and uronic acid (14.1-21.8%), with a small amount of proteins (3.9-9.3%), and included various levels of fucose (44.0-46.7%), mannose (18.9-26.8%), galactose (16.8-33.0%), xylose (10.7-17.0%) and glucose (3.5-9.5%). The crude and fractionated fucans contained one or two subfractions with average molecular weights (M-w) ranging from 110.1 x 10(3) to 2420 x 10(3) g/mol. The fractionated fucoidan, especially the F-1 fraction, strongly stimulated murine macrophages (Raw 264.7 cells), producing a considerable amount of nitric oxide (NO) and inducing expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and interleukin-10 (IL-10) transcripts by activation of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinases (MAPKs) pathways. The maximally immunoenhancing F1 fraction was mainly composed of (1 -> 3)-linked fucose, (1 -> 2)-linked mannose and (1 -> 4)-linked glucuronic acid with sulfates at C-2 or both the C-2 and C-4 positions in (1 -> 2,3)- and (1 -> 2,3,4)-linked fucose residues. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | UNDARIA-PINNATIFIDA | - |
dc.subject | STRUCTURAL-ANALYSIS | - |
dc.subject | ECKLONIA-CAVA | - |
dc.subject | IN-VITRO | - |
dc.subject | FUCOIDAN | - |
dc.subject | POLYSACCHARIDES | - |
dc.subject | CELLS | - |
dc.subject | SPOROPHYLL | - |
dc.subject | FRACTIONS | - |
dc.title | Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum | - |
dc.type | Article | - |
dc.citation.endPage | 514 | - |
dc.citation.startPage | 507 | - |
dc.citation.title | CARBOHYDRATE POLYMERS | - |
dc.citation.volume | 113 | - |
dc.contributor.alternativeName | 조명래 | - |
dc.identifier.bibliographicCitation | CARBOHYDRATE POLYMERS, v.113, pp.507 - 514 | - |
dc.identifier.doi | 10.1016/j.carbpol.2014.07.055 | - |
dc.identifier.scopusid | 2-s2.0-84907344277 | - |
dc.identifier.wosid | 000343613000063 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | UNDARIA-PINNATIFIDA | - |
dc.subject.keywordPlus | STRUCTURAL-ANALYSIS | - |
dc.subject.keywordPlus | ECKLONIA-CAVA | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | FUCOIDAN | - |
dc.subject.keywordPlus | POLYSACCHARIDES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SPOROPHYLL | - |
dc.subject.keywordPlus | FRACTIONS | - |
dc.subject.keywordAuthor | Agarum cribrosum | - |
dc.subject.keywordAuthor | Sulfated-fucans | - |
dc.subject.keywordAuthor | Immunomodulation | - |
dc.subject.keywordAuthor | Glycosidic linkage | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | MAPK | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |