Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum SCIE SCOPUS

Cited 36 time in WEB OF SCIENCE Cited 35 time in Scopus
Title
Molecular characterization and immunomodulatory activity of sulfated fucans from Agarum cribrosum
Author(s)
Cho, MyoungLae; Lee, Dong-Jin; Kim, Jin-Kyung; You, SangGuan
Publication Year
2014-11-26
Abstract
The sulfated-fucans, known as fucoidans, were isolated from Agarum cribrosum and fractionated using ion-exchange chromatography to determine their molecular characteristics and in vitro immunomodulatory activity. The crude and fractionated fucoidans (F-1 and F-2) consisted mostly of carbohydrates (52.4-56.0%), sulfates (12.7-23.0%) and uronic acid (14.1-21.8%), with a small amount of proteins (3.9-9.3%), and included various levels of fucose (44.0-46.7%), mannose (18.9-26.8%), galactose (16.8-33.0%), xylose (10.7-17.0%) and glucose (3.5-9.5%). The crude and fractionated fucans contained one or two subfractions with average molecular weights (M-w) ranging from 110.1 x 10(3) to 2420 x 10(3) g/mol. The fractionated fucoidan, especially the F-1 fraction, strongly stimulated murine macrophages (Raw 264.7 cells), producing a considerable amount of nitric oxide (NO) and inducing expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and interleukin-10 (IL-10) transcripts by activation of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinases (MAPKs) pathways. The maximally immunoenhancing F1 fraction was mainly composed of (1 -> 3)-linked fucose, (1 -> 2)-linked mannose and (1 -> 4)-linked glucuronic acid with sulfates at C-2 or both the C-2 and C-4 positions in (1 -> 2,3)- and (1 -> 2,3,4)-linked fucose residues. (C) 2014 Elsevier Ltd. All rights reserved.
ISSN
0144-8617
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2673
DOI
10.1016/j.carbpol.2014.07.055
Bibliographic Citation
CARBOHYDRATE POLYMERS, v.113, pp.507 - 514, 2014
Publisher
ELSEVIER SCI LTD
Subject
NF-KAPPA-B; UNDARIA-PINNATIFIDA; STRUCTURAL-ANALYSIS; ECKLONIA-CAVA; IN-VITRO; FUCOIDAN; POLYSACCHARIDES; CELLS; SPOROPHYLL; FRACTIONS
Keywords
Agarum cribrosum; Sulfated-fucans; Immunomodulation; Glycosidic linkage; NF-kappa B; MAPK
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER SCI LTD
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