CO에 반응하는 새로운 전사 조절자와 이를 이용한 T. onnurineus NA1의 수소 생산성 증가

Title
CO에 반응하는 새로운 전사 조절자와 이를 이용한 T. onnurineus NA1의 수소 생산성 증가
Alternative Title
A Novel CO-Responsive Transcriptional Regulator and Enhanced H2 Production by an Engineered Thermococcus onnurinues NA1
Author(s)
김민식; 최애란; 이성혁; 정해창; 배승섭; 양태준; 전정호; 임재규; 김태완; 윤환; 이현숙
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Jung, Hae Chang(정해창)Lee, Hyun Sook(이현숙)
Publication Year
2014-10-16
Abstract
Genome analysis of carboxydotrophic hydrogenogenic Thermococcus onnurineus NA1 revealed the existence of a putative transcriptional regulator system composed of CorQ with a V4R domain and CorR with a DNA-binding domain of LTTR family in close proximity to CO dehydrogenase (CODH) gene cluster. Homologous genes for the CorQR pairs were only found in Thermococcus species and Candidatus Korarchaeum cryptofilum OPF8. In-frame deletion of corQ or corR caused a severe impairment in CO-dependent growth and H2 production, suggesting that corQR encodes positive regulatory proteins essential for the expression of a CODH gene cluster. Introduction and expression of corQR genes under the control of a strong promoter in the corR-deletion strain, significantly increased mRNA and protein levels of the CODH gene relative to the wild-type strain. In a bioreactor culture, the ΔCorR/corQR↑ strain exhibited a 5.8-fold higher maximum H2 production rate than did the wild-type strain. To the best of our knowledge, this engineered strain has the highest H2 production rate, 191.9 mmol liter-1 h-1, and specific H2 production rate, 249.6 mmol g-1 h-1, higher than any CO-dependent H2-producing prokaryote reported so far. This study presents the first example of a transcriptional regulator system acting as a pair in the regulation of CO metabolism.lose proximity to CO dehydrogenase (CODH) gene cluster. Homologous genes for the CorQR pairs were only found in Thermococcus species and Candidatus Korarchaeum cryptofilum OPF8. In-frame deletion of corQ or corR caused a severe impairment in CO-dependent growth and H2 production, suggesting that corQR encodes positive regulatory proteins essential for the expression of a CODH gene cluster. Introduction and expression of corQR genes under the control of a strong promoter in the corR-deletion strain, significantly increased mRNA and protein levels of the CODH gene relative to the wild-type strain. In a bioreactor culture, the ΔCorR/corQR↑ strain exhibited a 5.8-fold higher maximum H2 production rate than did the wild-type strain. To the best of our knowledge, this engineered strain has the highest H2 production rate, 191.9 mmol liter-1 h-1, and specific H2 production rate, 249.6 mmol g-1 h-1, higher than any CO-dependent H2-producing prokaryote reported so far. This study presents the first example of a transcriptional regulator system acting as a pair in the regulation of CO metabolism.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25961
Bibliographic Citation
the 10th KSMB annual meeting and symposium, pp.50, 2014
Publisher
(사)해양바이오학회
Type
Conference
Language
English
Publisher
(사)해양바이오학회
Related Researcher
Research Interests

Marine extremophile,해양극한미생물

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