Identification of Pathogenic bacteria from Coral, Seaweed and Sponge

Title
Identification of Pathogenic bacteria from Coral, Seaweed and Sponge
Author(s)
김정희; 이애경; 원효경; 염승식; 우선옥
KIOST Author(s)
Lee, Na Yun(이나윤)Yum, Seungshic(염승식)
Publication Year
2014-11-07
Abstract
A few microorganisms have been identified as pathogenic agents responsible for various outbreaks of marine organism disease. But most of diseases have been implicated within a subset of marine organism in our knowledge of the host range and geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in coral, seaweed and sponge species using PCR-based assays. Total 5 pathogen-specific vibrio bacteria primer sets and 2 pathogen-specific 16s rDNA primer sets were designed for detecting coral, seaweed and sponge pathogenic disease. The pathogenic bacteria detected in coral were Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, Vibrio carchariae, and vibrio bacteria were detected in seaweed and sponge. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan, and to seaweed samples from Dokdo, Jeju, Yeosu and Uleongdo, and to sponge samples from Jeju in korea. After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in coral, seaweed and sponge. geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in coral, seaweed and sponge species using PCR-based assays. Total 5 pathogen-specific vibrio bacteria primer sets and 2 pathogen-specific 16s rDNA primer sets were designed for detecting coral, seaweed and sponge pathogenic disease. The pathogenic bacteria detected in coral were Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, Vibrio carchariae, and vibrio bacteria were detected in seaweed and sponge. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan, and to seaweed samples from Dokdo, Jeju, Yeosu and Uleongdo, and to sponge samples from Jeju in korea. After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in coral, seaweed and sponge.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25782
Bibliographic Citation
한국해양학회2014추계학술대회, pp.157, 2014
Publisher
(사)한국해양학회
Type
Conference
Language
English
Publisher
(사)한국해양학회
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