일산화탄소에 반응하는 새로운 타입의 전사조절자와 이를 기반으로한 엔지니어링을 통한 Thermococcus onnurineus NA1의 수소 생산성 증대

Title
일산화탄소에 반응하는 새로운 타입의 전사조절자와 이를 기반으로한 엔지니어링을 통한 Thermococcus onnurineus NA1의 수소 생산성 증대
Alternative Title
A Novel CO-Responsive Transcriptional Regulator and Enhanced H2 Production by an Engineered Thermococcus onnurineus NA1
Author(s)
김민식; 최애란; 이성혁; 정해창; 배승섭; 양태준; 전정호; 임재규; 윤환; 김태완; 이현숙; 강성균
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Jung, Hae Chang(정해창)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2015-01-20
Abstract
Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transcriptional regulator family in close proximity to the CO dehydrogenase (CODH) gene cluster. Homologous genes of the CorQR pair were also found in the genomes of Thermococcus species and “Candidatus Korarchaeum cryptofilum” OPF8. In-frame deletion of either corQ or corR caused a severe impairment in CO-dependent growth and H2 production. When corQ and corR deletion mutants were complemented by introducing the corQR genes under the control of a strong promoter, the mRNA and protein levels of the CODH gene were significantly increased in a CorR strain complemented with integrated corQR (CorR/corQR1) compared with those in the wild-type strain. In addition, the CorR/corQR1 strain exhibited a much higher H2 production rate (5.8-fold) than the wild-type strain in a bioreactor culture. The H2 production rate (191.9 mmol liter-1 h-1) and the specific H2 production rate (249.6 mmol g-1 h-1) of this strain were extremely high compared with those of CO-dependent H2-producing prokaryotes reported so far. These results suggest that the corQR genes encode a positive regulatory protein pair for the expression of a nyl reductase domain and CorR with a DNA-binding domain of the LysR-type transcriptional regulator family in close proximity to the CO dehydrogenase (CODH) gene cluster. Homologous genes of the CorQR pair were also found in the genomes of Thermococcus species and “Candidatus Korarchaeum cryptofilum” OPF8. In-frame deletion of either corQ or corR caused a severe impairment in CO-dependent growth and H2 production. When corQ and corR deletion mutants were complemented by introducing the corQR genes under the control of a strong promoter, the mRNA and protein levels of the CODH gene were significantly increased in a CorR strain complemented with integrated corQR (CorR/corQR1) compared with those in the wild-type strain. In addition, the CorR/corQR1 strain exhibited a much higher H2 production rate (5.8-fold) than the wild-type strain in a bioreactor culture. The H2 production rate (191.9 mmol liter-1 h-1) and the specific H2 production rate (249.6 mmol g-1 h-1) of this strain were extremely high compared with those of CO-dependent H2-producing prokaryotes reported so far. These results suggest that the corQR genes encode a positive regulatory protein pair for the expression of a
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25678
Bibliographic Citation
2015 한국 미생물 생명공학회 동계 심포지엄, pp.177, 2015
Type
Conference
Language
English
Related Researcher
Research Interests

Marine extremophile,해양극한미생물

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