Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon SCIE SCOPUS

Cited 10 time in WEB OF SCIENCE Cited 10 time in Scopus
Title
Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon
Author(s)
Jeon, Jeong Ho; Lim, Jae Kyu; Kim, Min-Sik; Yang, Tae-Jun; Lee, Seong-Hyuk; Bae, Seung Seob; Kim, Yun Jae; Lee, Sang Hee; Lee, Jung-Hyun; Kang, Sung Gyun; Lee, Hyun Sook
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2015-01
Abstract
The F-420-reducing hydrogenase has been known as a key enzyme in methanogenesis. Its homologs have been identified in non-methanogenic hyperthermophilic archaea, including Thermococcus onnurineus NA1, but neither physiological function nor biochemical properties have been reported to date. The enzyme of T. onnurineus NA1 was distinguished from those of other methanogens and the members of the family Desulfurobacteriaceae with respect to the phylogenetic distribution of the alpha and beta subunits, organization of frhAGB genes and conservation of F-420-coordinating residues. RT-qPCR and Western blot analyses revealed frhA gene is not silent but is expressed in T. onnurineus NA1 grown in the presence of sulfur, carbon monoxide, or formate. The trimeric enzyme complex was purified to homogeneity via affinity chromatography from T. onnurineus NA1 and exhibited catalytic activity toward the electron acceptors such as viologens and flavins but not the deazaflavin coenzyme F-420. This is the first biochemical study on the function of the frhAGB-encoding enzyme from a non-methanogenic archaea.
ISSN
1431-0651
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2563
DOI
10.1007/s00792-014-0689-y
Bibliographic Citation
EXTREMOPHILES, v.19, no.1, pp.109 - 118, 2015
Publisher
SPRINGER JAPAN KK
Keywords
F-420-reducing hydrogenase; Thermococcus onnurineus NA1; FrhAGB
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER JAPAN KK
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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