Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon
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Title
- Characterization of the frhAGB-encoding hydrogenase from a non-methanogenic hyperthermophilic archaeon
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Author(s)
- Jeon, Jeong Ho; Lim, Jae Kyu; Kim, Min-Sik; Yang, Tae-Jun; Lee, Seong-Hyuk; Bae, Seung Seob; Kim, Yun Jae; Lee, Sang Hee; Lee, Jung-Hyun; Kang, Sung Gyun; Lee, Hyun Sook
- KIOST Author(s)
- Lim, Jae Kyu(임재규); Lee, Seong Hyuk(이성혁); Kim, Yun Jae(김윤재); Lee, Jung Hyun(이정현); Kang, Sung Gyun(강성균); Lee, Hyun Sook(이현숙)
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Alternative Author(s)
- 임재규; 김민식; 양태준; 이성혁; 배승섭; 김윤재; 이정현; 강성균; 이현숙
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Publication Year
- 2015-01
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Abstract
- The F-420-reducing hydrogenase has been known as a key enzyme in methanogenesis. Its homologs have been identified in non-methanogenic hyperthermophilic archaea, including Thermococcus onnurineus NA1, but neither physiological function nor biochemical properties have been reported to date. The enzyme of T. onnurineus NA1 was distinguished from those of other methanogens and the members of the family Desulfurobacteriaceae with respect to the phylogenetic distribution of the alpha and beta subunits, organization of frhAGB genes and conservation of F-420-coordinating residues. RT-qPCR and Western blot analyses revealed frhA gene is not silent but is expressed in T. onnurineus NA1 grown in the presence of sulfur, carbon monoxide, or formate. The trimeric enzyme complex was purified to homogeneity via affinity chromatography from T. onnurineus NA1 and exhibited catalytic activity toward the electron acceptors such as viologens and flavins but not the deazaflavin coenzyme F-420. This is the first biochemical study on the function of the frhAGB-encoding enzyme from a non-methanogenic archaea.
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ISSN
- 1431-0651
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2563
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DOI
- 10.1007/s00792-014-0689-y
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Bibliographic Citation
- EXTREMOPHILES, v.19, no.1, pp.109 - 118, 2015
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Publisher
- SPRINGER JAPAN KK
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Keywords
- F-420-reducing hydrogenase; Thermococcus onnurineus NA1; FrhAGB
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Type
- Article
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Language
- English
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Document Type
- Article
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