Regulation of hydrogenase genes by sulfur-responsive regulator, SurR, in Thermococcus onnurineus NA1

Title
Regulation of hydrogenase genes by sulfur-responsive regulator, SurR, in Thermococcus onnurineus NA1
Author(s)
최애란; 김민식; 이성혁; 양태준; 김윤재; 이정현; 강성균; 이현숙
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2015-05-19
Abstract
SurR (sulfur response regulator) is a regulator which has been known to respond to elemental sulfur and to regulate hydrogenase genes in Pyrococcus furiosus. While P. furiosus contains only four hydrogenases (Mbh, Mbx, SulfI and SulfII), T. onnurineus NA1 has four more hydrogenases, Mfh1, Mfh2, Mch and Frh. T. onnurineus NA1 contains a SurR homolog and its binding site(GTTn3AAC) are conserved in the upstream region of 6 hydrogenase genes (frh, mbh, mbx, mfh2, sulfI, sulfII). To investigate the regulation of these hydrogenases by SurR, an in-frame deletion mutant of surR was constructed and the translation levels of hydrogenases were confirmed by western blotting. The regulation of expression of hydrogenases with SurR-binding sites was clearly demonstrated. Based on the expression pattern of hydrogenase genes, the role of SurR was determined as an activator or a repressor. Intriguingly, the expression pattern of sulfI and mbh in the presence of sulfur in T. onnurineus NA1 was different from that in P. furiosus. onnurineus NA1 has four more hydrogenases, Mfh1, Mfh2, Mch and Frh. T. onnurineus NA1 contains a SurR homolog and its binding site(GTTn3AAC) are conserved in the upstream region of 6 hydrogenase genes (frh, mbh, mbx, mfh2, sulfI, sulfII). To investigate the regulation of these hydrogenases by SurR, an in-frame deletion mutant of surR was constructed and the translation levels of hydrogenases were confirmed by western blotting. The regulation of expression of hydrogenases with SurR-binding sites was clearly demonstrated. Based on the expression pattern of hydrogenase genes, the role of SurR was determined as an activator or a repressor. Intriguingly, the expression pattern of sulfI and mbh in the presence of sulfur in T. onnurineus NA1 was different from that in P. furiosus.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25556
Bibliographic Citation
2015 극한미생물 국제심포지움, pp.31, 2015
Publisher
KIOST
Type
Conference
Language
English
Publisher
KIOST
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse