Rapid kit development for harmful algal detection

Title
Rapid kit development for harmful algal detection
Author(s)
이애경; 우선옥; 염승식
KIOST Author(s)
Lee, Na Yun(이나윤)Yum, Seungshic(염승식)
Publication Year
2015-05-20
Abstract
Invention of more quick, accurate, and efficient method which could have predictive and prognostic function is necessary to overcome the difficulties of conventional method for microorganisms monitoring. We have challenged to develop the immunochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The polyclonal antibody (pAb) against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense , which produces paralytic shellfish, was raised. The western blot analysis showed that the pAb detect three dinoflagellates, A. tamarense, H. pygmaea, and C. polykrikoides. But do not detect Akashiwo sanguinea. The pAb showed no signal in two diatom species, Cylindrotheca closterium, and Skeletonema costatum. Production of mAbs against A. tamarense RuBisCo large subunit is now under way for a rapid kit development.munochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The polyclonal antibody (pAb) against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense , which produces paralytic shellfish, was raised. The western blot analysis showed that the pAb detect three dinoflagellates, A. tamarense, H. pygmaea, and C. polykrikoides. But do not detect Akashiwo sanguinea. The pAb showed no signal in two diatom species, Cylindrotheca closterium, and Skeletonema costatum. Production of mAbs against A. tamarense RuBisCo large subunit is now under way for a rapid kit development.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25551
Bibliographic Citation
Harmful Algal Blooms and Climate Change, pp.30, 2015
Publisher
PICES
Type
Conference
Language
English
Publisher
PICES
Related Researcher
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