Rapid kit development for harmful algal detection

Abstract

Invention of more quick, accurate, and efficient method which could have predictive and prognostic function is necessary to overcome the difficulties of conventional method for microorganisms monitoring. We have challenged to develop the immunochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The polyclonal antibody (pAb) against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense , which produces paralytic shellfish, was raised. The western blot analysis showed that the pAb detect three dinoflagellates, A. tamarense, H. pygmaea, and C. polykrikoides. But do not detect Akashiwo sanguinea. The pAb showed no signal in two diatom species, Cylindrotheca closterium, and Skeletonema costatum. Production of mAbs against A. tamarense RuBisCo large subunit is now under way for a rapid kit development.munochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The polyclonal antibody (pAb) against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense , which produces paralytic shellfish, was raised. The western blot analysis showed that the pAb detect three dinoflagellates, A. tamarense, H. pygmaea, and C. polykrikoides. But do not detect Akashiwo sanguinea. The pAb showed no signal in two diatom species, Cylindrotheca closterium, and Skeletonema costatum. Production of mAbs against A. tamarense RuBisCo large subunit is now under way for a rapid kit development.