Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea SCIE SCOPUS KCI

Cited 13 time in WEB OF SCIENCE Cited 20 time in Scopus
Title
Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea
Author(s)
Kim, Ji Hyung; Abu Affan, Md; Jang, Jiyi; Kang, Mee-Hye; Ko, Ah-Ra; Jeon, Seon-Mi; Oh, Chulhong; Heo, Soo-Jin; Lee, Youn-Ho; Ju, Se-Jong; Kang, Do-Hyung
KIOST Author(s)
Oh, Chul Hong(오철홍)Heo, Soo Jin(허수진)Lee, Youn Ho(이윤호)Kang, Do Hyung(강도형)
Publication Year
2015-02
Abstract
A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 +/- 0.2%, 48.8 +/- 1.8%, and 22.2 +/- 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.
ISSN
1017-7825
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2542
DOI
10.4014/jmb.1410.10032
Bibliographic Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.2, pp.238 - 246, 2015
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Subject
RNA GENE-SEQUENCES; ONCORHYNCHUS-MYKISS; PHAFFIA-RHODOZYMA; RAINBOW-TROUT; PLUVIALIS; CAROTENOIDS; 18S; PHYLOGENY; PIGMENTS; PURIFICATION
Keywords
Haematococcus sp KORDI03; astaxanthin; bioresource production; phylogenetic analysis
Type
Article
Language
English
Document Type
Article
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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