OPTIMIZATION OF CULTURE CONDITION FOR BIOMASS PRODUCTION OF SALINE Tetraselmis sp. WITH ENHANCING FATTY ACIDS CONCENTRATION

Abstract

Biodiesel production from microalgae has received much attention and microalgal lipid can be converted to biodiesel since the lipid is mainly composed by saturated and unsaturated fatty acids [1, 2]. Among the many species in microalgae, saline Tetraselmis sp. is primarily having high amount of total lipid. The aim of this study is to maintain the biomass production of Tetraselmis sp. Ganghwa KCTC 12236BP with enhancing lipid concentration using modified f/2 media (f/2, f and f*1.5 media) under the different culture volume regimes (5 L Flask, 200 L plastic tank and 20 ton raceway pond) for 14-days culture experiments. Furthermore, we analyzed the total lipid content and delivertized fatty acid methyl esters (FAMEs) using AOAC method [3] and GC-MASS, respectively. In the 7-days duration of 5 L Flask culture condition, the average biomass of the species in f*1.5, f and f/2 media were estimated to be 0.42 g/L, 0.30 g/L and 0.23 g/L, respectively. The final average biomass of the species in f*1.5 medium were significantly peaked up to 0.65 g/L. Moreover, the 14-days biomass productivities under 200 L and 20 ton scales were estimated to be 0.21 g/L and 0.31 g/L under the f*1.5 medium, respectively. Biomass productivity in 200 L scale was lower than 5 L scale while the total lipid content was higher (21.2%) than that of 5 L scale (17.0%). FAMEs were analyzed to be 89.9%, 89.0% and 90.3% cultured in 5 L, 200 L and 20 ton line Tetraselmis sp. is primarily having high amount of total lipid. The aim of this study is to maintain the biomass production of Tetraselmis sp. Ganghwa KCTC 12236BP with enhancing lipid concentration using modified f/2 media (f/2, f and f*1.5 media) under the different culture volume regimes (5 L Flask, 200 L plastic tank and 20 ton raceway pond) for 14-days culture experiments. Furthermore, we analyzed the total lipid content and delivertized fatty acid methyl esters (FAMEs) using AOAC method [3] and GC-MASS, respectively. In the 7-days duration of 5 L Flask culture condition, the average biomass of the species in f*1.5, f and f/2 media were estimated to be 0.42 g/L, 0.30 g/L and 0.23 g/L, respectively. The final average biomass of the species in f*1.5 medium were significantly peaked up to 0.65 g/L. Moreover, the 14-days biomass productivities under 200 L and 20 ton scales were estimated to be 0.21 g/L and 0.31 g/L under the f*1.5 medium, respectively. Biomass productivity in 200 L scale was lower than 5 L scale while the total lipid content was higher (21.2%) than that of 5 L scale (17.0%). FAMEs were analyzed to be 89.9%, 89.0% and 90.3% cultured in 5 L, 200 L and 20 ton