One step nucleic acid extraction and amplification on an integrated microdevice for early dection of Cochlodinium polykrikoides

Abstract

Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell &#8211 Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea &#8211 was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection. harmful algal blooms in South Korea &#8211 was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection.