One step nucleic acid extraction and amplification on an integrated microdevice for early dection of Cochlodinium polykrikoides
DC Field | Value | Language |
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dc.contributor.author | T.P.O. Nguyen | - |
dc.contributor.author | 정승원 | - |
dc.contributor.author | K.T.L. Trinh | - |
dc.contributor.author | B.M. Tran | - |
dc.contributor.author | Q.N. Pham | - |
dc.contributor.author | J.H. Kim | - |
dc.contributor.author | N.Y. Lee | - |
dc.date.accessioned | 2020-07-15T23:33:32Z | - |
dc.date.available | 2020-07-15T23:33:32Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2015-10-29 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/25208 | - |
dc.description.abstract | Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell – Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea – was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection. harmful algal blooms in South Korea – was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into subsequent PCR channel. Gram-negative Escherichia coli bacterium was used to illustrate the adhesion phenomenon based on its similar negatively-charged surface in comparison with algal cells. A 244-bp amplicon from the genome of C. polykrikoides algae was successfully amplified using this microdevice, demonstrating its potential application on further on-site analysis for early algal detection. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | MicroTAS | - |
dc.relation.isPartOf | MicroTAS | - |
dc.title | One step nucleic acid extraction and amplification on an integrated microdevice for early dection of Cochlodinium polykrikoides | - |
dc.type | Conference | - |
dc.citation.conferencePlace | US | - |
dc.citation.endPage | 3 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | MicroTAS | - |
dc.contributor.alternativeName | 정승원 | - |
dc.identifier.bibliographicCitation | MicroTAS, pp.1 - 3 | - |
dc.description.journalClass | 1 | - |