Medium optimization for enhancement of formate-driven bio-hydrogen production by the hyperthemophilic archaeon, Thermococcus onnurineus NA1

Title
Medium optimization for enhancement of formate-driven bio-hydrogen production by the hyperthemophilic archaeon, Thermococcus onnurineus NA1
Author(s)
김태완; 이성목; 이현숙; 이정현; 나정걸; 강성균
KIOST Author(s)
Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2015-12-23
Abstract
The hyperthermophilic archaeon, Thermococcus onnurineus NA1 which was isolated from deep-sea hydrothermal vent area has been reported to be able to grow on formate evolving hydrogen (H2) in a growth-associated manner. In this study, we optimized the composition of medium to enhance the formate-driven H2 production by T. onnurineus NA1 by using response surface methodology (RSM). Of the minor components of medium including potassium, magnesium, vitamin and trace elements, based on the Plackett-Burman design, trace elements was selected as the only positively effective component affecting H2 production. Subsequently, the optimal concentrations of the trace elements and the major components of medium such as NaCl, yeast extract and sodium formate were determined by the five-level central composite design. As a result, the maximum H2 concentration of 31.7%, which was 20% enhanced value compared with that obtained using control medium, was predicted when the concentrations of sodium formate, NaCl, yeast extract and trace elements were 11.31 g/L, 27.36 g/L, 4.01 g/L and 5.31 mL/L, respectively. In control medium, 10 g/L of sodium formate, 35 g/L of NaCl, 1 g/L of yeast extract and 1 mL/L of trace elements were included. The result was further verified by triplicate batch cultivation in serum bottle based on the optimized medium showing the practicability of this optimum strategy.mized the composition of medium to enhance the formate-driven H2 production by T. onnurineus NA1 by using response surface methodology (RSM). Of the minor components of medium including potassium, magnesium, vitamin and trace elements, based on the Plackett-Burman design, trace elements was selected as the only positively effective component affecting H2 production. Subsequently, the optimal concentrations of the trace elements and the major components of medium such as NaCl, yeast extract and sodium formate were determined by the five-level central composite design. As a result, the maximum H2 concentration of 31.7%, which was 20% enhanced value compared with that obtained using control medium, was predicted when the concentrations of sodium formate, NaCl, yeast extract and trace elements were 11.31 g/L, 27.36 g/L, 4.01 g/L and 5.31 mL/L, respectively. In control medium, 10 g/L of sodium formate, 35 g/L of NaCl, 1 g/L of yeast extract and 1 mL/L of trace elements were included. The result was further verified by triplicate batch cultivation in serum bottle based on the optimized medium showing the practicability of this optimum strategy.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24969
Bibliographic Citation
2015 International Chemical Congress of Pacific Basin Societies, pp.218, 2015
Publisher
Pacific Basin Societies
Type
Conference
Language
English
Publisher
Pacific Basin Societies
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse