Adaptive evolution of a hyperthermophilic archaeon Thermococcus onnurineus NA1 during growth on formate

Title
Adaptive evolution of a hyperthermophilic archaeon Thermococcus onnurineus NA1 during growth on formate
Author(s)
정해창; 이성혁; 이정현; 이현숙; 강성균
KIOST Author(s)
Jung, Hae Chang(정해창)Lee, Seong Hyuk(이성혁)Lee, Jung Hyun(이정현)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2016-04-20
Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 has been reported to grow on formate coupled with H¬ 2 production and ATP generation (HCOO- + H2O → HCO3- + H2, ΔG80oC = -2.6 kJ/mol). In an attempt to develop the strain for the enhanced H2 production on formate, adaptive evolution was adopted. T. onnurineus NA1 was grown to stationary phase to trigger spontaneous mutation and then transferred to a fresh medium containing formate. Through serial transfers of batch cultures, physiological changes were monitored and gradual increases in cell density, H2 production rate and formate consumption rate were observed. After 156 transfers, the evolved strain showed 1.4-, 1.3- and 1.3-fold higher values in maximum cell density, H2 production rate and formate consumption rate, respectively, in comparison with those of parental strain. In order to obtain an integrative understanding of the genetic and phenotypic changes during evolution on formate, genome sequencing was performed using the PacBio Single Molecule Real-Time (SMRT) sequencing technology. As a result, we discovered eleven mutation sites, including insertion (2 sites), deletion (2 sites) and substitution (7 sites) either at the intergenic (2 sites) or coding regions (9 sites) in a DNA sequence. Further mutational studies would be required to assess the contribution of gene mutations to the phenotypic changes during evolution. The potential of the enginehe enhanced H2 production on formate, adaptive evolution was adopted. T. onnurineus NA1 was grown to stationary phase to trigger spontaneous mutation and then transferred to a fresh medium containing formate. Through serial transfers of batch cultures, physiological changes were monitored and gradual increases in cell density, H2 production rate and formate consumption rate were observed. After 156 transfers, the evolved strain showed 1.4-, 1.3- and 1.3-fold higher values in maximum cell density, H2 production rate and formate consumption rate, respectively, in comparison with those of parental strain. In order to obtain an integrative understanding of the genetic and phenotypic changes during evolution on formate, genome sequencing was performed using the PacBio Single Molecule Real-Time (SMRT) sequencing technology. As a result, we discovered eleven mutation sites, including insertion (2 sites), deletion (2 sites) and substitution (7 sites) either at the intergenic (2 sites) or coding regions (9 sites) in a DNA sequence. Further mutational studies would be required to assess the contribution of gene mutations to the phenotypic changes during evolution. The potential of the engine
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24896
Bibliographic Citation
한국미생물학회지/ 한국미생물학회, pp.195, 2016
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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