Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis

Title
Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis
Author(s)
이성혁; 김태완; 이성목; 정해창; 최애란; 이정현; 권개경; 이현숙; 강성균; 김민식
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Jung, Hae Chang(정해창)Lee, Jung Hyun(이정현)Kwon, Kae Kyoung(권개경)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2016-10-06
Abstract
Adaptive laboratory evolution is one of engineering approaches to produce new and fit phenotypes by long-term selection under a defined condition. To understand the molecular mechanisms driving adaptive phenotypic change, systems-level analysis such as comparative whole-genome sequencing and polyomic analysis has been performed. In this study, we evolved a hyperthermophilic archaeon Thermococcus onnurineus NA1 through serial transfers of a culture to fresh medium with carbonmonoxide (CO) over 100 times. The changes in the phenotype, genome sequence and transcriptome were analyzed. The growth rate, CO consumption rate and H2 production rate of the strain gradually increased with transfer time. The genome of evolved strains was sequenced and several mutations were identified in comparison with a parental strain. The gene expression pattern obtained by transcriptome analysis was consistent with the phenotypic changes, and some genes involved in COutilization such as carbon monoxide dehydrogenase, hydrogenase, Na+/H+-antiporter and ATP synthetase were significantly upregulated along with the adaption. This study demonstrates for the first time that CO-utilizing ability of microbes canbe improved by adaptive evolutionary approach.ysis such as comparative whole-genome sequencing and polyomic analysis has been performed. In this study, we evolved a hyperthermophilic archaeon Thermococcus onnurineus NA1 through serial transfers of a culture to fresh medium with carbonmonoxide (CO) over 100 times. The changes in the phenotype, genome sequence and transcriptome were analyzed. The growth rate, CO consumption rate and H2 production rate of the strain gradually increased with transfer time. The genome of evolved strains was sequenced and several mutations were identified in comparison with a parental strain. The gene expression pattern obtained by transcriptome analysis was consistent with the phenotypic changes, and some genes involved in COutilization such as carbon monoxide dehydrogenase, hydrogenase, Na+/H+-antiporter and ATP synthetase were significantly upregulated along with the adaption. This study demonstrates for the first time that CO-utilizing ability of microbes canbe improved by adaptive evolutionary approach.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24572
Bibliographic Citation
2016 Asian Biohydrogen & Biogas Symposium, pp.106, 2016
Publisher
2016
Type
Conference
Language
English
Publisher
2016
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse