Cloning of Cytosine N4-methyltransferases from Marine Alphabacteria

Title
Cloning of Cytosine N4-methyltransferases from Marine Alphabacteria
Author(s)
김정희; 권개경; 오현명
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Publication Year
2017-04-27
Abstract
DNA methylation is involved in a diversity of processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, has N6-Adenine or N4-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Methylation patterns of Celeribacter marinus IMCC12053 and Novosphingobium pentaromativorans US6-1 were compared. Both strains have been observed to change adenosine of 5-GANTC-3’as N6-methyladenosine, and N4-cytosine of 5-CpG-3’(IMCC12053) and 5-GpC-3’(US6-1) as N4-methylcytosine. Using phylogenetic analysis exocylic DNA methyltransferases from both of the species were chosen for cloning. In this study cloned exocyclic exocyclic DNA methylases are presented, and the potential use of novel type of CpG and GpC methylases in molecular biology and epigenetics.4-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Methylation patterns of Celeribacter marinus IMCC12053 and Novosphingobium pentaromativorans US6-1 were compared. Both strains have been observed to change adenosine of 5-GANTC-3’as N6-methyladenosine, and N4-cytosine of 5-CpG-3’(IMCC12053) and 5-GpC-3’(US6-1) as N4-methylcytosine. Using phylogenetic analysis exocylic DNA methyltransferases from both of the species were chosen for cloning. In this study cloned exocyclic exocyclic DNA methylases are presented, and the potential use of novel type of CpG and GpC methylases in molecular biology and epigenetics.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24020
Bibliographic Citation
2017한국미생물학회 국제학술대회, pp.191, 2017
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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