Cloning of Cytosine N4-methyltransferases from Marine Alphabacteria

DC Field Value Language
dc.contributor.author 김정희 -
dc.contributor.author 권개경 -
dc.contributor.author 오현명 -
dc.date.accessioned 2020-07-15T15:52:16Z -
dc.date.available 2020-07-15T15:52:16Z -
dc.date.created 2020-02-11 -
dc.date.issued 2017-04-27 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/24020 -
dc.description.abstract DNA methylation is involved in a diversity of processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, has N6-Adenine or N4-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Methylation patterns of Celeribacter marinus IMCC12053 and Novosphingobium pentaromativorans US6-1 were compared. Both strains have been observed to change adenosine of 5-GANTC-3’as N6-methyladenosine, and N4-cytosine of 5-CpG-3’(IMCC12053) and 5-GpC-3’(US6-1) as N4-methylcytosine. Using phylogenetic analysis exocylic DNA methyltransferases from both of the species were chosen for cloning. In this study cloned exocyclic exocyclic DNA methylases are presented, and the potential use of novel type of CpG and GpC methylases in molecular biology and epigenetics.4-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Methylation patterns of Celeribacter marinus IMCC12053 and Novosphingobium pentaromativorans US6-1 were compared. Both strains have been observed to change adenosine of 5-GANTC-3’as N6-methyladenosine, and N4-cytosine of 5-CpG-3’(IMCC12053) and 5-GpC-3’(US6-1) as N4-methylcytosine. Using phylogenetic analysis exocylic DNA methyltransferases from both of the species were chosen for cloning. In this study cloned exocyclic exocyclic DNA methylases are presented, and the potential use of novel type of CpG and GpC methylases in molecular biology and epigenetics. -
dc.description.uri 2 -
dc.language English -
dc.publisher 한국미생물학회 -
dc.relation.isPartOf 2017한국미생물학회 국제학술대회 -
dc.title Cloning of Cytosine N4-methyltransferases from Marine Alphabacteria -
dc.type Conference -
dc.citation.conferencePlace KO -
dc.citation.endPage 191 -
dc.citation.startPage 191 -
dc.citation.title 2017한국미생물학회 국제학술대회 -
dc.contributor.alternativeName 권개경 -
dc.identifier.bibliographicCitation 2017한국미생물학회 국제학술대회, pp.191 -
dc.description.journalClass 2 -
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Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 2. Conference Papers
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