Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1

Title
Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1
Author(s)
나혜윤; 유수정; 이연주; 권개경
KIOST Author(s)
Lee, Yeon Ju(이연주)Kwon, Kae Kyoung(권개경)
Publication Year
2017-04-27
Abstract
Novosphingobium pentaromativorans US6-1 is a marine bacterium that was isolated from muddy sediments of Ulsan Bay, Korea and could utilize high-molecular-weight PAHs as a sole carbon and energy source. Degradation of dibenzofuran (DBF) by strain US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process.train US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24012
Bibliographic Citation
한국미생물학회 2017 국제학술대회, pp.200, 2017
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

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