Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1
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Title
- Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1
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Author(s)
- 나혜윤; 유수정; 이연주; 권개경
- KIOST Author(s)
- Na, Hye Yun(나혜윤); Lee, Yeon Ju(이연주); Kwon, Kae Kyoung(권개경)
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Alternative Author(s)
- 나혜윤; 유수정; 이연주; 권개경
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Publication Year
- 2017-04-27
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Abstract
- Novosphingobium pentaromativorans US6-1 is a marine bacterium that was isolated from muddy sediments of Ulsan Bay, Korea and could utilize high-molecular-weight PAHs as a sole carbon and energy source. Degradation of dibenzofuran (DBF) by strain US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process.train US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/24012
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Bibliographic Citation
- 한국미생물학회 2017 국제학술대회, pp.200, 2017
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Publisher
- 한국미생물학회
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Type
- Conference
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Language
- English
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