Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 나혜윤 | - |
dc.contributor.author | 유수정 | - |
dc.contributor.author | 이연주 | - |
dc.contributor.author | 권개경 | - |
dc.date.accessioned | 2020-07-15T15:33:59Z | - |
dc.date.available | 2020-07-15T15:33:59Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2017-04-27 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/24012 | - |
dc.description.abstract | Novosphingobium pentaromativorans US6-1 is a marine bacterium that was isolated from muddy sediments of Ulsan Bay, Korea and could utilize high-molecular-weight PAHs as a sole carbon and energy source. Degradation of dibenzofuran (DBF) by strain US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process.train US6-1 was compared with Sphingomonas wittichii RW1 - known as the outstanding DBF-degrading bacterium - faster than that by strain RW1. Phylogenetic analysis of α-submits of the aromatic ring hydrogenase of the strains US6-1 and RW1 with other strains revealed that genes of the strain US6-1 were differentially clustered from those of strain RW1 which degrade DBF via angular dioxygenation process. Degradation of DBF by strain US6-1 was monitored with fluorescence spectrometer and metabolic intermediates were analyzed by GC-MS and LC-MS. As a result, 3(2H)-benzofuranone was detected and this implied that degradation of DBF by strain US6-1 was started with lateral dioxygenation process. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | 한국미생물학회 2017 국제학술대회 | - |
dc.title | Studies on the Pathway and Enzymes Involved in the Degradation of Dibenzofuran by Novosphingobium pentaromativorans US6-1 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 200 | - |
dc.citation.startPage | 200 | - |
dc.citation.title | 한국미생물학회 2017 국제학술대회 | - |
dc.contributor.alternativeName | 나혜윤 | - |
dc.contributor.alternativeName | 유수정 | - |
dc.contributor.alternativeName | 이연주 | - |
dc.contributor.alternativeName | 권개경 | - |
dc.identifier.bibliographicCitation | 한국미생물학회 2017 국제학술대회, pp.200 | - |
dc.description.journalClass | 2 | - |