LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과
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Title
- LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과
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Alternative Title
- Inhibitory effect of Heterosigma akashiwo extracts on activation of lipopolysaccharide-stimulated mouse macrophage RAW264. 7 cells
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Author(s)
- 김준성; 최윤경; 예보람; 김은아; 허수진
- KIOST Author(s)
- Kim, Jun Seong(김준성); Kim, Eun A(김은아); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김준성; 최윤경; 예보람; 김은아; 허수진
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Publication Year
- 2017-10-02
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Abstract
- Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the Raphidophyceae, causes much damage to all marine ecosystem. Reduce damage to marine ecosystems want to use as a resource. In this study, Ethyl acetate fraction-Methanol fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/23793
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Bibliographic Citation
- KSBMB International Connference 2017, pp.108, 2017
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Publisher
- KSBMB
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Type
- Conference
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Language
- English
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