LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과

Title
LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과
Alternative Title
Inhibitory effect of Heterosigma akashiwo extracts on activation of lipopolysaccharide-stimulated mouse macrophage RAW264. 7 cells
Author(s)
김준성; 최윤경; 예보람; 김은아; 허수진
KIOST Author(s)
Kim, Junseong(김준성)Kim, Eun A(김은아)Heo, Soo Jin(허수진)
Publication Year
2017-10-02
Abstract
Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the Raphidophyceae, causes much damage to all marine ecosystem. Reduce damage to marine ecosystems want to use as a resource. In this study, Ethyl acetate fraction-Methanol fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23793
Bibliographic Citation
KSBMB International Connference 2017, pp.108, 2017
Publisher
KSBMB
Type
Conference
Language
English
Publisher
KSBMB
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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