LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과
DC Field | Value | Language |
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dc.contributor.author | 김준성 | - |
dc.contributor.author | 최윤경 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 김은아 | - |
dc.contributor.author | 허수진 | - |
dc.date.accessioned | 2020-07-15T14:34:10Z | - |
dc.date.available | 2020-07-15T14:34:10Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2017-10-02 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/23793 | - |
dc.description.abstract | Red tide Heterosigma akashiwo (H. akashiwo), a microscopic alga of the Raphidophyceae, causes much damage to all marine ecosystem. Reduce damage to marine ecosystems want to use as a resource. In this study, Ethyl acetate fraction-Methanol fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent.fraction (HAEM) obtained from H. akashiwo using organic solvent fractionation and then evaluated anti-inflammatory activity in LPS-stimulated RAW 264.7 cells and zebrafish model. The result exhibited that HAME80 markedly inhibited the productions of nitric oxide (NO) and prostaglandin E2 (PGE2). Also, HAEM80 down-regulated the protein expression levels of inducible nitric oxide synthase (iNOS) and Cyclooxygenase (COX-2) as well as the secretion of interleukin-1β (IL-1β) in LPS-stimulated RAW 264.7 cells. In further study, HAME80 improved the survival rate and decreased the yolk edema in LPS-treated zebrafish embryo. Moreover, HAME80 significantly reduced the production of ROS and NO and the cell death induced by LPS- stimulated in zebrafish embryo. The peak was confirmed 1to20 by gas chromatography mass spectrometry (GC-MS). Therefore, we suggest that H. akashiwo could be used as a beneficial anti-inflammatory agent. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | KSBMB | - |
dc.relation.isPartOf | KSBMB International Connference 2017 | - |
dc.title | LPS로 염증을 유도한 RAW264.7 세포에서 Heterosigma akashiwo의 항염증 효과 | - |
dc.title.alternative | Inhibitory effect of Heterosigma akashiwo extracts on activation of lipopolysaccharide-stimulated mouse macrophage RAW264. 7 cells | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 108 | - |
dc.citation.startPage | 108 | - |
dc.citation.title | KSBMB International Connference 2017 | - |
dc.contributor.alternativeName | 김준성 | - |
dc.contributor.alternativeName | 최윤경 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 김은아 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.identifier.bibliographicCitation | KSBMB International Connference 2017, pp.108 | - |
dc.description.journalClass | 1 | - |