Effect of micronutrients on growth of blue-green alga, Spirulina (Arthrospira) maxima using a ratio of inorganic components of Jeju magma seawater

Abstract

Jeju magma seawater (underground seawater, JMS) contains high amount of micronutrients more than that of general seawater or deep ocean water. JMS especially has Zinc (Z), Iron (F), Manganese (M), Vanadium (V), Selenium (S) and Germanium (G) as much as 10 times in comparison to general seawater. Limited information has been reported on the relation between JMS and growth of marine algae. In addition, growth of Spirulina maxima using micronutrients of JMS has not been conducted yet. The purpose of this study was to find out the effect of the growth of S. maxima with the micronutrients of JMS. Experiment for the growth rate of S. maxima was accomplished by modified 64 SOT media of each micronutrient combinations for fourteen days using spectrophotometric measurement (λ=664 nm). Micronutrients ratio of the culture media were made each concentration same as JMS. Spirulina maxima growth rate in the media containing M and V showed highest absorbance at 14 days, following Z+M+V, Z+F+M, M and M+G combinations. In contrast, the medium of Z+M+S+G recorded the lowest absorbance, following Z+V+S+G, Z+F+V+S, Z+F+S+G and F+V. Long-term scale-up study should be needed for the study of accelerating growth mechanism with biochemical analysis of S. maxima cells in order to determine the effect on micronutrients of M, V, Z, F, G and S.) as much as 10 times in comparison to general seawater. Limited information has been reported on the relation between JMS and growth of marine algae. In addition, growth of Spirulina maxima using micronutrients of JMS has not been conducted yet. The purpose of this study was to find out the effect of the growth of S. maxima with the micronutrients of JMS. Experiment for the growth rate of S. maxima was accomplished by modified 64 SOT media of each micronutrient combinations for fourteen days using spectrophotometric measurement (λ=664 nm). Micronutrients ratio of the culture media were made each concentration same as JMS. Spirulina maxima growth rate in the media containing M and V showed highest absorbance at 14 days, following Z+M+V, Z+F+M, M and M+G combinations. In contrast, the medium of Z+M+S+G recorded the lowest absorbance, following Z+V+S+G, Z+F+V+S, Z+F+S+G and F+V. Long-term scale-up study should be needed for the study of accelerating growth mechanism with biochemical analysis of S. maxima cells in order to determine the effect on micronutrients of M, V, Z, F, G and S.