ANTI-INFLAMMATORY EFFECT OF SUPERCRITICAL EXTRACT AND ITS CONSTITUENTS FROM ISHIGE OKAMURAE SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
ANTI-INFLAMMATORY EFFECT OF SUPERCRITICAL EXTRACT AND ITS CONSTITUENTS FROM ISHIGE OKAMURAE
Author(s)
Ko, Eun-Yi; Yoon, Weon-Jong; Lee, Hae-Won; Heo, Soo-Jin; Ko, Young-Hwan; Fernando, I. P. Shanura; Cho, Kichul; Lee, Chi-Heon; Hur, Sung-Pyo; Cho, Su-Hyeon; Ahn, Ginnae; Kim, Daekyung; Kim, Kil-Nam
KIOST Author(s)
Heo, Soo Jin(허수진)Hur, Sung Pyo(허성표)
Publication Year
2016
Abstract
The anti-inflammatory properties of the supercritical fluid extract of Ishige okamurae (SFEIO) on lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. The lipid profile of the SFEIO, reviled the presence of palmitic acid (220.2 mg/g), linoleic acid (168.0 mg/g), and oleic acid (123.0 mg/g). SFEIO was found to exert it's anti-inflammatory effects through inhibiting nitric oxide (NO), prostaglandin E-2 (PGE(2)), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 production in LPS-stimulated RAW 264.7 cells, without inducing cytotoxicity. SFEIO did not effect on the LPS-induced p38 kinase phosphorylation, whereas it attenuated the extracellular-related signaling kinase (ERK) and c-Jun N-terminal kinase (JNK) phosphorylation. Furthermore, SFEIO inhibited the LPS-induced I kappa B-alpha degradation and p50 NF-kappa B activation. These results suggest that SFEIO exerts its anti-inflammatory effects in LPSactivated RAW 264.7 cells by down-regulating the activation of ERK, JNK, and NF-kappa B.
ISSN
1611-2156
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2324
DOI
10.17179/excli2016-337
Bibliographic Citation
EXCLI JOURNAL, v.15, pp.434 - 445, 2016
Publisher
EXCLI JOURNAL MANAGING OFFICE
Subject
NF-KAPPA-B; NITRIC-OXIDE SYNTHASE; RAW 264.7 CELLS; FATTY-ACIDS; TRANSCRIPTION FACTOR; MURINE MACROPHAGES; SIGNALING PATHWAYS; FLUID EXTRACTION; SKELETAL-MUSCLE; OLEIC-ACID
Keywords
supercritical fluid extraction; linoleic acid; Ishige okamurae; anti-inflammatory; NF-kappa B
Type
Article
Language
English
Document Type
Article
Publisher
EXCLI JOURNAL MANAGING OFFICE
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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