Test of anaerobic fluorescent protein as reporter of gene expression in Clostridium sp. AWRP

Title
Test of anaerobic fluorescent protein as reporter of gene expression in Clostridium sp. AWRP
Author(s)
도미숙; 이종민; 채철기; 권수재; 김윤재; 강성균; 이현숙
KIOST Author(s)
Lee, Joungmin(이종민)Kwon, Soo Jae(권수재)Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)
Publication Year
2018-10-04
Abstract
Acetogenic bacteria are important industrial microorganisms that produce various chemicals and fuels. Effective genetic tools are still needed for improved chemical production through metabolic engineering. Anaerobic fluorescent protein evoglow as reporter gene is used to monitor gene expression in some anaerobes. In this study, we tested evoglow expression under phosphotransbutyrylase promoter (Pptb) of C. acetobutylicum in Clostridium sp. AWRP, a newly isolated acetogenic species. The recombinant expression vectors were introduced into Clostridium sp. AWRP via conjugation. The expression was analyzed by fluorescent microscopy. Here, we showed that the fluorescence intensity was strong in Clostridium sp. AWRP codon- optimized evoglow gene. These results suggest that the anaerobic fluorescent protein evoglow can be used for effective monitoring gene expression in Clostridium sp. AWRP.glow as reporter gene is used to monitor gene expression in some anaerobes. In this study, we tested evoglow expression under phosphotransbutyrylase promoter (Pptb) of C. acetobutylicum in Clostridium sp. AWRP, a newly isolated acetogenic species. The recombinant expression vectors were introduced into Clostridium sp. AWRP via conjugation. The expression was analyzed by fluorescent microscopy. Here, we showed that the fluorescence intensity was strong in Clostridium sp. AWRP codon- optimized evoglow gene. These results suggest that the anaerobic fluorescent protein evoglow can be used for effective monitoring gene expression in Clostridium sp. AWRP.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23109
Bibliographic Citation
DSM, International Workshop on Deep-Sea Microbiology, pp.53, 2018
Publisher
KIOST
Type
Conference
Language
English
Publisher
KIOST
Related Researcher
Research Interests

Metabolic engineering,Marine microbiology,대사공학,해양미생물

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