Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP
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Title
- Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP
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Author(s)
- 권수재; 이종민; 강성균; 이현숙
- KIOST Author(s)
- Kwon, Soo Jae(권수재); Lee, Joungmin(이종민); Kang, Sung Gyun(강성균); Lee, Hyun Sook(이현숙)
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Alternative Author(s)
- 권수재; 이종민; 강성균; 이현숙
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Publication Year
- 2018-10-04
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Abstract
- Some acetogenic bacteria has the ability to use carbon monoxide (CO) using the Wood-Ljungdahl pathway. Biological conversion of CO using acetogens offers benefits of utilizing industrial waste gases containing CO and generating valuable fuels such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis.ls such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/23108
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Bibliographic Citation
- DSM (2018 International Workshop on Deep-Sea Microbiology), 2018
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Publisher
- 한국해양과학기술원,
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Type
- Conference
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Language
- English
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