Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP

DC Field Value Language
dc.contributor.author 권수재 -
dc.contributor.author 이종민 -
dc.contributor.author 강성균 -
dc.contributor.author 이현숙 -
dc.date.accessioned 2020-07-15T10:53:53Z -
dc.date.available 2020-07-15T10:53:53Z -
dc.date.created 2020-02-11 -
dc.date.issued 2018-10-04 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/23108 -
dc.description.abstract Some acetogenic bacteria has the ability to use carbon monoxide (CO) using the Wood-Ljungdahl pathway. Biological conversion of CO using acetogens offers benefits of utilizing industrial waste gases containing CO and generating valuable fuels such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis.ls such as ethanol, butanol and 2,3-butanediol. In this study, we screened 2,3-butanediol-producing microbes among various environmental samples and isolated a new strain, Clostridium sp. AWRP from a wetland. This strain is able to produce acetate, ethanol and 2,3-butanediol as major metabolites using CO. To improve 2,3-butanediol production, the wild-type strain was modified by random mutagenesis technology. After treatment of cells with N-methyl-N-nitro-N-nitrosoguanidine (NTG), mutants with better growth than the wild-type strain under high CO partial pressure were selected. They showed different growth and metabolite production patterns. Among them, a few mutants were identified to have improved CO consumption and 2,3-butanediol production. Here, we describe effective strategies for selection of CO-utilizing 2,3-butanediol producers through enrichment processes during isolation and mutagenesis. -
dc.description.uri 1 -
dc.language English -
dc.publisher 한국해양과학기술원, -
dc.relation.isPartOf DSM (2018 International Workshop on Deep-Sea Microbiology) -
dc.title Improvement of 2,3-butanediol production from CO gas by random mutagenesis of Clostridium sp. AWRP -
dc.type Conference -
dc.citation.conferenceDate 2018-10 -
dc.citation.conferencePlace KO -
dc.citation.title DSM (2018 International Workshop on Deep-Sea Microbiology) -
dc.contributor.alternativeName 권수재 -
dc.contributor.alternativeName 이종민 -
dc.contributor.alternativeName 강성균 -
dc.contributor.alternativeName 이현숙 -
dc.identifier.bibliographicCitation DSM (2018 International Workshop on Deep-Sea Microbiology) -
dc.description.journalClass 1 -
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Marine Resources & Environment Research Division > Marine Biotechnology &Bioresource Research Department > 2. Conference Papers
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