Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation

Title
Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation
Author(s)
이종민
KIOST Author(s)
Lee, Joungmin(이종민)
Publication Year
2018-10-26
Abstract
Synthesis gas (Syngas) fermentation has been attracting attention since it can be prepared by gasification of various materials that can be directly utilized by microbes. Previously, we isolated a novel alcohologenic acetogen Clostridium sp. AWRP at Ansan. This bacterium is able to produce ethanol and 2,3-butanediol from either CO or CO2 plus H2, but as stated above, engineering this species had been hampered as well by recalcitrance against DNA transformation and non-optimized culture conditions. In this study, we introduce a recent progress in domestication of the AWRP strain: establishment of a genetic toolbox and improvement of culture conditions. In the first part, we discuss construction of Clostridium-E. coli shuttle plasmids and protocols of their transfer to the AWRP strain. The second part consists of modifications of culture medium, focusing on effects of trace elements and gas transfer.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22951
Bibliographic Citation
2018 Fall Symposium of the Korean Institute of Chemical Engineers, pp.104, 2018
Publisher
한국화학공학회/C1 가스 리파이너리 사업단
Type
Conference
Language
English
Publisher
한국화학공학회/C1 가스 리파이너리 사업단
Related Researcher
Research Interests

Metabolic engineering,Marine microbiology,대사공학,해양미생물

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