Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation
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Title
- Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation
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Author(s)
- 이종민
- KIOST Author(s)
- Lee, Joungmin(이종민)
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Alternative Author(s)
- 이종민
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Publication Year
- 2018-10-26
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Abstract
- Synthesis gas (Syngas) fermentation has been attracting attention since it can be prepared by gasification of various materials that can be directly utilized by microbes. Previously, we isolated a novel alcohologenic acetogen Clostridium sp. AWRP at Ansan. This bacterium is able to produce ethanol and 2,3-butanediol from either CO or CO2 plus H2, but as stated above, engineering this species had been hampered as well by recalcitrance against DNA transformation and non-optimized culture conditions. In this study, we introduce a recent progress in domestication of the AWRP strain: establishment of a genetic toolbox and improvement of culture conditions. In the first part, we discuss construction of Clostridium-E. coli shuttle plasmids and protocols of their transfer to the AWRP strain. The second part consists of modifications of culture medium, focusing on effects of trace elements and gas transfer.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/22951
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Bibliographic Citation
- 2018 Fall Symposium of the Korean Institute of Chemical Engineers, pp.104, 2018
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Publisher
- 한국화학공학회/C1 가스 리파이너리 사업단
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Type
- Conference
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Language
- English
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