Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이종민 | - |
dc.date.accessioned | 2020-07-15T10:32:23Z | - |
dc.date.available | 2020-07-15T10:32:23Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2018-10-26 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/22951 | - |
dc.description.abstract | Synthesis gas (Syngas) fermentation has been attracting attention since it can be prepared by gasification of various materials that can be directly utilized by microbes. Previously, we isolated a novel alcohologenic acetogen Clostridium sp. AWRP at Ansan. This bacterium is able to produce ethanol and 2,3-butanediol from either CO or CO2 plus H2, but as stated above, engineering this species had been hampered as well by recalcitrance against DNA transformation and non-optimized culture conditions. In this study, we introduce a recent progress in domestication of the AWRP strain: establishment of a genetic toolbox and improvement of culture conditions. In the first part, we discuss construction of Clostridium-E. coli shuttle plasmids and protocols of their transfer to the AWRP strain. The second part consists of modifications of culture medium, focusing on effects of trace elements and gas transfer. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | 한국화학공학회/C1 가스 리파이너리 사업단 | - |
dc.relation.isPartOf | 2018 Fall Symposium of the Korean Institute of Chemical Engineers | - |
dc.title | Domestication of Clostridium sp. AWRP towards a Platform Strain for Syngas Fermentation | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 104 | - |
dc.citation.startPage | 104 | - |
dc.citation.title | 2018 Fall Symposium of the Korean Institute of Chemical Engineers | - |
dc.contributor.alternativeName | 이종민 | - |
dc.identifier.bibliographicCitation | 2018 Fall Symposium of the Korean Institute of Chemical Engineers, pp.104 | - |
dc.description.journalClass | 1 | - |