The rapid kit and its digital reader for harmful algal detection
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Title
- The rapid kit and its digital reader for harmful algal detection
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Author(s)
- 염승식; 신경순; 백승호
- KIOST Author(s)
- Yum, Seung Shic(염승식); Shin, Kyoung Soon(신경순); Baek, Seung Ho(백승호)
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Alternative Author(s)
- 염승식; 신경순; 백승호
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Publication Year
- 2018-10-30
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Abstract
- We have challenged to develop the immunochromatography based rapid kit for microalgae detection for developing
more quick, accurate, and efficient method which could have predictive and prognostic function to overcome the
difficulties of conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal
antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species
in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of
H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate
carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces
paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction
of microalgal cells in a sample.conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal
antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species
in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of
H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate
carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces
paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction
of microalgal cells in a sample.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/22939
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Bibliographic Citation
- PICES-2018 Annual Meeting, pp.190, 2018
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Publisher
- PICES
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Type
- Conference
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Language
- English
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