The rapid kit and its digital reader for harmful algal detection

Title
The rapid kit and its digital reader for harmful algal detection
Author(s)
염승식; 신경순; 백승호
KIOST Author(s)
Yum, Seungshic(염승식)Shin, Kyoungsoon(신경순)Baek, Seung Ho(백승호)
Publication Year
2018-10-30
Abstract
We have challenged to develop the immunochromatography based rapid kit for microalgae detection for developing
more quick, accurate, and efficient method which could have predictive and prognostic function to overcome the
difficulties of conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal
antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species
in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of
H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate
carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces
paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction
of microalgal cells in a sample.conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal
antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species
in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of
H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate
carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces
paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction
of microalgal cells in a sample.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22939
Bibliographic Citation
PICES-2018 Annual Meeting, pp.190, 2018
Publisher
PICES
Type
Conference
Language
English
Publisher
PICES
Related Researcher
Research Interests

Marine Ecology,Marine Environment,HABs,해양생태,해양환경,적조

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