The rapid kit and its digital reader for harmful algal detection

Abstract

We have challenged to develop the immunochromatography based rapid kit for microalgae detection for developing more quick, accurate, and efficient method which could have predictive and prognostic function to overcome the difficulties of conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction of microalgal cells in a sample.conventional method for microorganisms monitoring. As a result, a rapid kit using monoclonal antibodies (mAbs) raised against α-tubulin of Heterocapsa triquetra, a candidate of harmful algal bloom species in the coast of Korea. The rapid kit showed a positive signal at about 5,000 cells of H. triquetra 50,000 cells of H. pygmaea and Cochlodinium polykrikoides and so on. The mAb against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit and Photosystem I subunit 9 of Alexandrium tamarense, which produces paralytic shellfish, were also raised. We also developed a digital reader for the rapid kit for quantitative prediction of microalgal cells in a sample.