Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer SCIE SCOPUS

Cited 25 time in WEB OF SCIENCE Cited 25 time in Scopus
Title
Adverse Effects, Expression of the Bk-CYP3045C1 Gene, and Activation of the ERK Signaling Pathway in the Water Accommodated Fraction-Exposed Rotifer
Author(s)
Won, Eun-Ji; Kim, Ryeo-Ok; Kang, Hye-Min; Kim, Hui-Su; Hwang, Dae-Sik; Han, Jeonghoon; Lee, Young Hwan; Hwang, Un-Ki; Zhou, Bingsheng; Lee, Su-Jae; Lee, Jae-Seong
Publication Year
2016-06-07
Abstract
To examine the deleterious effects of the water accommodated fraction (WAF) of crude oil, the growth curve, fecundity, and lifespan of the monogonont rotifer (Brachionus koreanus) were measured for 24 h in response to three different doses (0.2X, 0.4X, and 0.8X) of WAFs. A higher dose of WAFs significantly reduced the fecundity and lifespan. A rotifer 32K microarray chip showed that the Bk-CYP3045C1 gene had the highest expression. Of the 25 entire CYP genes, the Bk-CYP3045C1 gene showed a significant expression for different doses and times in response to WAFs and chemical components of WAFs (naphthalene and phenanthrene); also, glutathione S-transferase genes, ABC transporter, and other genes showed dose responses upon exposure to 80% WAF over time. Different doses of WAFs increased the oxidative stress with an induction of reactive oxygen species (ROS) and a depletion of glutathione (GSH). Exposure to WAFs did not show toxic effects on survivability in B. koreanus; however, toxicity to WAFs was shown when piperonyl butoxide, a potent inhibitor of cytochrome P450 (CYP) enzymes, was added. This toxicity was dose-dependent. After WAFs exposure, PERK was activated over time in response to WAFs, which suggests that WAFs can be activated by the p-ERK signaling pathway.
ISSN
0013-936X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2272
DOI
10.1021/acs.est.6b01306
Bibliographic Citation
ENVIRONMENTAL SCIENCE & TECHNOLOGY, v.50, no.11, pp.6025 - 6035, 2016
Publisher
AMER CHEMICAL SOC
Subject
POLYCYCLIC AROMATIC-HYDROCARBONS; CRUDE-OIL; BRACHIONUS-KOREANUS; MONOGONONT ROTIFER; CYTOCHROME-P450 CYP; OXIDATIVE STRESS; PROTEIN-KINASES; DISPERSED OIL; TOXICITY; SPILL
Type
Article
Language
English
Document Type
Article
Publisher
AMER CHEMICAL SOC
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