Large quantities secretion to culture medium with epidermal growth factor and trigger factor fused protein in Escherichia coli

Title
Large quantities secretion to culture medium with epidermal growth factor and trigger factor fused protein in Escherichia coli
Author(s)
강윤혁; 조은영; Sachithra Amarin Hettiarachchi; 오철홍; 김태호
KIOST Author(s)
Kang, Yoon Hyeok(강윤혁)Jo, Eunyoung(조은영)Oh, Chulhong(오철홍)Kim, Taeho(김태호)
Publication Year
2019-07-11
Abstract
Recombinant protein secretion system to periplasmic space or culture medium in E. coli is beneficial for protein folding improvement, low proteolytic activity and easy downstream process. In this study, we designed and synthesized the xylanase signal sequence (originated from Bacillus subtilis)-human epidermal growth factor-E. coli trigger factor fused protein (XSP-hEGF-TF) gene for expression the protein in E. coli periplasm. The synthesized gene was cloned into pET11a expression vector and expressed in E. coli BL21(DE3). Previously reported that xylanase signal peptide (XSP) is known to move target proteins into periplasmic space in E. coli. However, we observed that the XSP-hEGF-TF protein is secreted in large quantities (700 mg/liter) to culture medium. The reason maybe the effect of signal peptide, combinations of the proteins, or unknown effects. In our other study, XSP moved the target protein (human superoxide desmutase) to periplasmic space in E. coli. Addtional experiments are needed to find out the reasons behind this phenomenon. Then it will be beneficial for simplified downstream purification process and to increase the potential of efficient protein production method.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/22553
Bibliographic Citation
New Approaches and Concepts in Microbiology, pp.155, 2019
Publisher
EMBO|EMBL
Type
Conference
Language
English
Publisher
EMBO|EMBL
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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