Optimal culture method to improve the productivity of Haematococcus sp.KORDI03

Optimal culture method to improve the productivity of Haematococcus sp.KORDI03
유용균; 이원규; 김태호; 강도형
KIOST Author(s)
Ryu, Yong Kyun(유용균)Lee, Won Kyu(이원규)Kim, Taeho(김태호)Kang, Do-Hyung(강도형)
Publication Year
Haematococcus sp. are well known for the ability to synthesize and accumulate of the red ketocarotenoid astaxanthin (3,39-dihydroxy-b,b-caratene-4,49-dione) for up to 4% of the total cellular dry weight under induced stress condition (nutrient deprivation, increased salinity and exposure to high temperature). Astaxanthin is a high value-added product with applications in pharmaceuicals, nutraceuticals, and animal nutrition. However, the culture cost (nutrients, water, land and system etc.) are considerably high, it is essential to develop an affordable culture condition for mass production and sustainability of the strain. In this reason, the purpose of this study was to develop for reducing culture cost by invented culture method and to examine an optimal condition for biomass production of the Haematococcus sp. KORDI03 (KCTC 12348BP). The broth-cultured strain (3.2x106 cells/mL, 0.0012 g/L) was inoculated by different conditions of inoculationvolume and spray feed interval on BBM medium-based plates (Ø90 x 25 x duplicate) with room temperature (28±0.5°C) and fluorescence light (12L: 12D cycle) conditions during a month. Red cyst cells were observed from 10 days after inoculation (over 50% confluence), and the final production of dried red cyst cells were ranged from 1.63 g/m2 (5 mL inoculation vol. and 5 day-fed interval) to 21.12 g/m2 (1 mL inoculation vol. and 1 day-fed interval).
Bibliographic Citation
제 33회 한국조류학회 학술발표대회 및 정기총회, pp.73, 2019
사단법인 한국조류학회
사단법인 한국조류학회
Related Researcher
Research Interests

Microalgae Culture and Application,Aquaculture System Engineering,미세조류 배양 및 응용,배양시스템 엔지니어링,유용해양생물 배양 및 응용

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