Detection of coat protein gene of nervous necrosis virus using loop mediated isothermal amplification SCIE SCOPUS

Cited 9 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Detection of coat protein gene of nervous necrosis virus using loop mediated isothermal amplification
Author(s)
Hwang, Jinik; Suh, Sung-Suk; Park, Mirye; Oh, Myung-Joo; Kim, Jong-Oh; Lee, Sukchan; Lee, Taek-Kyun
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2016-03
Abstract
Objective: To establish a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the identification of nervous necrosis virus (NNV) infection. Methods: A set of synthesized primers was used to match the sequences of a specific region of the nnv gene from the National Center for Biotechnology Information database, not originating from NNV-infected fish, the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and time. In addition, to determine species-specific LAMP primers, cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine birnavirus. Results: The optimized LAMP reaction carried out at 64 degrees C for 60 loin, and above 4 U Bst DNA polymerase. The sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain reaction. The LAMP assay primers were specific for the detection NNV infection in Epinephelus septemfasciatus. Conclusions: The development of LAMP primers based on genetic information from a public database, not virus-infected samples. may provide a very simple and convenient method to identify viral infection in aquatic organisms.
ISSN
1995-7645
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2216
DOI
10.1016/j.apjtm.2016.01.035
Bibliographic Citation
ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, v.9, no.3, pp.230 - 235, 2016
Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
Subject
BETANODAVIRUS INFECTIONS; FISH; DIAGNOSIS; SEA
Keywords
Nervous necrosis virus; Nodaviridae; Polymerase chain reaction; Loop-mediated isothermal; Amplification
Type
Article
Language
English
Document Type
Article
Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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