Detection of coat protein gene of nervous necrosis virus using loop mediated isothermal amplification SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Hwang, Jinik | - |
dc.contributor.author | Suh, Sung-Suk | - |
dc.contributor.author | Park, Mirye | - |
dc.contributor.author | Oh, Myung-Joo | - |
dc.contributor.author | Kim, Jong-Oh | - |
dc.contributor.author | Lee, Sukchan | - |
dc.contributor.author | Lee, Taek-Kyun | - |
dc.date.accessioned | 2020-04-20T02:40:23Z | - |
dc.date.available | 2020-04-20T02:40:23Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2016-03 | - |
dc.identifier.issn | 1995-7645 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/2216 | - |
dc.description.abstract | Objective: To establish a novel and highly specific loop-mediated isothermal amplification (LAMP) assay for the identification of nervous necrosis virus (NNV) infection. Methods: A set of synthesized primers was used to match the sequences of a specific region of the nnv gene from the National Center for Biotechnology Information database, not originating from NNV-infected fish, the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and time. In addition, to determine species-specific LAMP primers, cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine birnavirus. Results: The optimized LAMP reaction carried out at 64 degrees C for 60 loin, and above 4 U Bst DNA polymerase. The sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain reaction. The LAMP assay primers were specific for the detection NNV infection in Epinephelus septemfasciatus. Conclusions: The development of LAMP primers based on genetic information from a public database, not virus-infected samples. may provide a very simple and convenient method to identify viral infection in aquatic organisms. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | WOLTERS KLUWER MEDKNOW PUBLICATIONS | - |
dc.subject | BETANODAVIRUS INFECTIONS | - |
dc.subject | FISH | - |
dc.subject | DIAGNOSIS | - |
dc.subject | SEA | - |
dc.title | Detection of coat protein gene of nervous necrosis virus using loop mediated isothermal amplification | - |
dc.type | Article | - |
dc.citation.endPage | 235 | - |
dc.citation.startPage | 230 | - |
dc.citation.title | ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | - |
dc.citation.volume | 9 | - |
dc.citation.number | 3 | - |
dc.contributor.alternativeName | 황진익 | - |
dc.contributor.alternativeName | 서승석 | - |
dc.contributor.alternativeName | 박미례 | - |
dc.contributor.alternativeName | 이택견 | - |
dc.identifier.bibliographicCitation | ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, v.9, no.3, pp.230 - 235 | - |
dc.identifier.doi | 10.1016/j.apjtm.2016.01.035 | - |
dc.identifier.scopusid | 2-s2.0-84960119898 | - |
dc.identifier.wosid | 000369038600005 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | BETANODAVIRUS INFECTIONS | - |
dc.subject.keywordPlus | FISH | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | SEA | - |
dc.subject.keywordAuthor | Nervous necrosis virus | - |
dc.subject.keywordAuthor | Nodaviridae | - |
dc.subject.keywordAuthor | Polymerase chain reaction | - |
dc.subject.keywordAuthor | Loop-mediated isothermal | - |
dc.subject.keywordAuthor | Amplification | - |
dc.relation.journalWebOfScienceCategory | Public, Environmental & Occupational Health | - |
dc.relation.journalWebOfScienceCategory | Tropical Medicine | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Public, Environmental & Occupational Health | - |
dc.relation.journalResearchArea | Tropical Medicine | - |