Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis
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Title
- Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis
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Author(s)
- Lee, Seong Hyuk; Kim, Min-Sik; Lee, Jae-Hak; Kim, Tae Wan; Bae, Seung Seob; Lee, Sung-Mok; Jung, Hae Chang; Yang, Tae-Jun; Choi, Ae Ran; Cho, Yong-Jun; Lee, Jung-Hyun; Kwon, Kae Kyoung; Lee, Hyun Sook; Kang, Sung Gyun
- KIOST Author(s)
- Lee, Seong Hyuk(이성혁); Lee, Sung Mok(이성목); Lee, Jung Hyun(이정현); Kwon, Kae Kyoung(권개경); Lee, Hyun Sook(이현숙); Kang, Sung Gyun(강성균)
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Alternative Author(s)
- 이성혁; 김태완; 이성목; 정해창; 양태준; 이정현; 권개경; 이현숙; 강성균
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Publication Year
- 2016-03-15
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Abstract
- The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H-2 on carbon monoxide (CO) and its H-2 production rates have been improved through metabolic engineering. In this study, we applied adaptive evolution to enhance H-2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H-2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H-2 productivity at high CO flow rates using synthesis gas produced from coal gasification.
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ISSN
- 2045-2322
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2212
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DOI
- 10.1038/srep22896
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Bibliographic Citation
- SCIENTIFIC REPORTS, v.6, 2016
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Publisher
- NATURE PUBLISHING GROUP
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Subject
- THERMOCOCCUS-ONNURINEUS NA1; DIFFERENTIAL EXPRESSION ANALYSIS; DEPENDENT H-2 PRODUCTION; SEA HYDROTHERMAL VENT; STATIONARY-PHASE; HYDROGEN-PRODUCTION; ESCHERICHIA-COLI; DNA METHYLATION; SPONTANEOUS MUTATION; SINGLE-MOLECULE
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Type
- Article
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Language
- English
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Document Type
- Article
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