Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis SCIE SCOPUS

Cited 13 time in WEB OF SCIENCE Cited 15 time in Scopus
Title
Adaptive engineering of a hyperthermophilic archaeon on CO and discovering the underlying mechanism by multi-omics analysis
Author(s)
Lee, Seong Hyuk; Kim, Min-Sik; Lee, Jae-Hak; Kim, Tae Wan; Bae, Seung Seob; Lee, Sung-Mok; Jung, Hae Chang; Yang, Tae-Jun; Choi, Ae Ran; Cho, Yong-Jun; Lee, Jung-Hyun; Kwon, Kae Kyoung; Lee, Hyun Sook; Kang, Sung Gyun
KIOST Author(s)
Lee, Seong Hyuk(이성혁)Jung, Hae Chang(정해창)Lee, Jung Hyun(이정현)Kwon, Kae Kyoung(권개경)Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)
Publication Year
2016-03-15
Abstract
The hyperthermophilic archaeon Thermococcus onnurineus NA1 can grow and produce H-2 on carbon monoxide (CO) and its H-2 production rates have been improved through metabolic engineering. In this study, we applied adaptive evolution to enhance H-2 productivity. After over 150 serial transfers onto CO medium, cell density, CO consumption rate and H-2 production rate increased. The underlying mechanism for those physiological changes could be explained by using multi-omics approaches including genomic, transcriptomic and epigenomic analyses. A putative transcriptional regulator was newly identified to regulate the expression levels of genes related to CO oxidation. Transcriptome analysis revealed significant changes in the transcript levels of genes belonging to the categories of transcription, translation and energy metabolism. Our study presents the first genome-scale methylation pattern of hyperthermophilic archaea. Adaptive evolution led to highly enhanced H-2 productivity at high CO flow rates using synthesis gas produced from coal gasification.
ISSN
2045-2322
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2212
DOI
10.1038/srep22896
Bibliographic Citation
SCIENTIFIC REPORTS, v.6, 2016
Publisher
NATURE PUBLISHING GROUP
Subject
THERMOCOCCUS-ONNURINEUS NA1; DIFFERENTIAL EXPRESSION ANALYSIS; DEPENDENT H-2 PRODUCTION; SEA HYDROTHERMAL VENT; STATIONARY-PHASE; HYDROGEN-PRODUCTION; ESCHERICHIA-COLI; DNA METHYLATION; SPONTANEOUS MUTATION; SINGLE-MOLECULE
Type
Article
Language
English
Document Type
Article
Publisher
NATURE PUBLISHING GROUP
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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