Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes
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Title
- Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes
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Author(s)
- Kang, Min-Cheol; Ding, Yuling; Kim, Eun-A; Choi, Youn Kyung; De Araujo, Thiago; Heo, Soo-Jin; Lee, Seung-Hong
- KIOST Author(s)
- Kim, Eun A(김은아); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김은아; 최윤경; 허수진
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Publication Year
- 2017-04
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Abstract
- Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives (STCs)-indole-2-carboxaldehyde (STC-1), indole-3-carboxaldehyde (STC-2), indole-4-carboxaldehyde (STC-3), indole-5-carboxaldehyde (STC-4), indole-6-carboxaldehyde (STC-5), and indole-7-carboxaldehyde (STC-6)-from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells. We found that STC-1 and STC-5 resulted in non-toxic inhibition of the differentiation of 3T3-L1 adipocytes and thus selected these compounds for further study. STC-1 and STC-5 significantly inhibited lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT/enhancer-binding protein alpha (C/EBP alpha), and sterol regulatory element-binding protein 1c (SREBP-1c) in a dose-dependent manner. The specific mechanism mediating the effects of STC-1 and STC-5 was shown to be AMP-activated protein kinase (AMPK) activation. Our results demonstrated the inhibitory effect of STC-1 and STC-5 on adipogenesis through the activation of the AMPK signal pathway. Together, these findings suggested that STC-1 and STC-5 may be effective candidates for the prevention of obesity or obesity-related diseases.
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ISSN
- 1660-3397
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2125
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DOI
- 10.3390/md15040119
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Bibliographic Citation
- MARINE DRUGS, v.15, no.4, 2017
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Publisher
- MDPI AG
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Subject
- GENE-EXPRESSION; ECKLONIA-CAVA; DIFFERENTIATION; MECHANISMS; SEAWEED; DISEASE; OBESITY; CELLS; DIECKOL; MICE
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Keywords
- anti-obesity effect; adipocyte; Sargassum thunbergii; indole derivatives
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Type
- Article
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Language
- English
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Document Type
- Article
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