Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes SCIE SCOPUS

Cited 26 time in WEB OF SCIENCE Cited 28 time in Scopus
Title
Indole Derivatives Isolated from Brown Alga Sargassum thunbergii Inhibit Adipogenesis through AMPK Activation in 3T3-L1 Preadipocytes
Author(s)
Kang, Min-Cheol; Ding, Yuling; Kim, Eun-A; Choi, Youn Kyung; De Araujo, Thiago; Heo, Soo-Jin; Lee, Seung-Hong
KIOST Author(s)
Kim, Eun A(김은아)Heo, Soo Jin(허수진)
Publication Year
2017-04
Abstract
Seaweed, a popular and abundant food ingredient mainly consumed in Asian countries, is a good source of bioactive compounds with anti-obesity effects. However, the anti-obesity effects of Sargassum thunbergii have not yet been established. In this study, we isolated six indole derivatives (STCs)-indole-2-carboxaldehyde (STC-1), indole-3-carboxaldehyde (STC-2), indole-4-carboxaldehyde (STC-3), indole-5-carboxaldehyde (STC-4), indole-6-carboxaldehyde (STC-5), and indole-7-carboxaldehyde (STC-6)-from S. thunbergii and evaluated their inhibitory effects on adipocyte differentiation in 3T3-L1 cells. We found that STC-1 and STC-5 resulted in non-toxic inhibition of the differentiation of 3T3-L1 adipocytes and thus selected these compounds for further study. STC-1 and STC-5 significantly inhibited lipid accumulation and downregulated the expression of peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT/enhancer-binding protein alpha (C/EBP alpha), and sterol regulatory element-binding protein 1c (SREBP-1c) in a dose-dependent manner. The specific mechanism mediating the effects of STC-1 and STC-5 was shown to be AMP-activated protein kinase (AMPK) activation. Our results demonstrated the inhibitory effect of STC-1 and STC-5 on adipogenesis through the activation of the AMPK signal pathway. Together, these findings suggested that STC-1 and STC-5 may be effective candidates for the prevention of obesity or obesity-related diseases.
ISSN
1660-3397
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2125
DOI
10.3390/md15040119
Bibliographic Citation
MARINE DRUGS, v.15, no.4, 2017
Publisher
MDPI AG
Subject
GENE-EXPRESSION; ECKLONIA-CAVA; DIFFERENTIATION; MECHANISMS; SEAWEED; DISEASE; OBESITY; CELLS; DIECKOL; MICE
Keywords
anti-obesity effect; adipocyte; Sargassum thunbergii; indole derivatives
Type
Article
Language
English
Document Type
Article
Publisher
MDPI AG
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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