A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1
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Title
- A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1
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Author(s)
- Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Hyun Sook; Lee, Jung-Hyun
- KIOST Author(s)
- Kim, Yun Jae(김윤재); Lim, Jae Kyu(임재규); Kang, Sung Gyun(강성균); Lee, Hyun Sook(이현숙); Lee, Jung Hyun(이정현)
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Alternative Author(s)
- 이규비; 김윤재; 임재규; 강성균; 이현숙; 이정현
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Publication Year
- 2019-04
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Abstract
- The biosynthetic pathway of 2,3-butanediol (2,3-BDO) production from pyruvate under anaerobic conditions includes three enzymes: acetolactate synthase (ALS), acetolactate decarboxylase (ALDC), and acetoin reductase (AR). Recently, in anaerobic hyperthermophilic Pyrococcus furiosus, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from pyruvate by ALS through a temperature-dependent metabolic switch. In this study, we first attempted to produce 2,3-BDO from Thermococcus onnurineus NA1 using a simple biosynthetic pathway by two enzymes (ALS and AR) at a high temperature. Two heterologous genes, acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from T. guaymacensis, were introduced and expressed in T. onnurineus NA1. The mutant strain produced approximately 3.3 mM 2,3-BDO at 80 degrees C. An acetyl-CoA synthetase III (TON_1001) was further deleted to enhance 2,3-BDO production, and the mutant strain showed a 25% increase in the specific production of 2,3-BDO. Furthermore, when carbon monoxide (CO) gas was added as a reductant, specific production of 2,3-BDO increased by 45%. These results suggest a new biosynthetic pathway for 2,3-BDO and demonstrate the possibility of T. onnurineus NA1 as a platform strain for 2,3-BDO production at high temperatures.
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ISSN
- 0175-7598
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2052
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DOI
- 10.1007/s00253-019-09724-z
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Bibliographic Citation
- APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.103, no.8, pp.3477 - 3485, 2019
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Publisher
- SPRINGER
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Subject
- DEPENDENT H-2 PRODUCTION; CLOSTRIDIUM-LJUNGDAHLII; PYROCOCCUS-FURIOSUS; ACETOIN; 3-HYDROXYPROPIONATE; DEHYDROGENASE; SYNTHETASE; SEQUENCE; DELETION; REMOVAL
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Keywords
- 2; 3-Butanediol; Hyperthermophile; Metabolic engineering; Thermococcus onnurineus NA1
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Type
- Article
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Language
- English
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Document Type
- Article
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