A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 1 time in WEB OF SCIENCE Cited 1 time in Scopus
Title
A simple biosynthetic pathway for 2,3-butanediol production in Thermococcus onnurineus NA1
Author(s)
Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Hyun Sook; Lee, Jung-Hyun
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)
Publication Year
2019-04
Abstract
The biosynthetic pathway of 2,3-butanediol (2,3-BDO) production from pyruvate under anaerobic conditions includes three enzymes: acetolactate synthase (ALS), acetolactate decarboxylase (ALDC), and acetoin reductase (AR). Recently, in anaerobic hyperthermophilic Pyrococcus furiosus, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from pyruvate by ALS through a temperature-dependent metabolic switch. In this study, we first attempted to produce 2,3-BDO from Thermococcus onnurineus NA1 using a simple biosynthetic pathway by two enzymes (ALS and AR) at a high temperature. Two heterologous genes, acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from T. guaymacensis, were introduced and expressed in T. onnurineus NA1. The mutant strain produced approximately 3.3 mM 2,3-BDO at 80 degrees C. An acetyl-CoA synthetase III (TON_1001) was further deleted to enhance 2,3-BDO production, and the mutant strain showed a 25% increase in the specific production of 2,3-BDO. Furthermore, when carbon monoxide (CO) gas was added as a reductant, specific production of 2,3-BDO increased by 45%. These results suggest a new biosynthetic pathway for 2,3-BDO and demonstrate the possibility of T. onnurineus NA1 as a platform strain for 2,3-BDO production at high temperatures.
ISSN
0175-7598
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2052
DOI
10.1007/s00253-019-09724-z
Bibliographic Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.103, no.8, pp.3477 - 3485, 2019
Publisher
SPRINGER
Subject
DEPENDENT H-2 PRODUCTION; CLOSTRIDIUM-LJUNGDAHLII; PYROCOCCUS-FURIOSUS; ACETOIN; 3-HYDROXYPROPIONATE; DEHYDROGENASE; SYNTHETASE; SEQUENCE; DELETION; REMOVAL
Keywords
2; 3-Butanediol; Hyperthermophile; Metabolic engineering; Thermococcus onnurineus NA1
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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