Nine co-localized cytochrome P450 genes of the CYP2N, CYP2AD, and CYP2P gene families in the mangrove killifish Kryptolebias marmoratus genome: Identification and expression in response to B[Α]P, BPA, OP, and NP SCIE SCOPUS

Cited 8 time in WEB OF SCIENCE Cited 8 time in Scopus
Title
Nine co-localized cytochrome P450 genes of the CYP2N, CYP2AD, and CYP2P gene families in the mangrove killifish Kryptolebias marmoratus genome: Identification and expression in response to B[Α]P, BPA, OP, and NP
Author(s)
Puthumana, J.; Kim, B.-M.; Jeong, C.-B.; Kim, D.-H.; Kang, H.-M.; Jung, J.-H.; Kim, I.-C.; Hwang, U.-K.; Lee, J.-S.
KIOST Author(s)
Jung, Jee Hyun(정지현)
Publication Year
2017
Abstract
The CYP2 genes are the largest and most diverse cytochrome P450 (CYP) subfamily in vertebrates. We have identified nine co-localized CYP2 genes (∼55 kb) in a new cluster in the genome of the highly resilient ecotoxicological fish model Kryptolebias marmoratus. Molecular characterization, temporal and tissue-specific expression pattern, and response to xenobiotics of these genes were examined. The CYP2 gene clusters were characterized and designated CYP2N22-23, CYP2AD12, and CYP2P16-20. Gene synteny analysis confirmed that the cluster in K. marmoratus is similar to that found in other teleost fishes, including zebrafish. A gene duplication event with diverged catalytic function was observed in CYP2AD12. Moreover, a high level of divergence in expression was observed among the co-localized genes. Phylogeny of the cluster suggested an orthologous relationship with similar genes in zebrafish and Japanese medaka. Gene expression analysis showed that CYP2P19 and CYP2N20 were consecutively expressed throughout embryonic development, whereas CYP2P18 was expressed in all adult tissues, suggesting that members of each CYP2 gene family have different physiological roles even though they are located in the same cluster. Among endocrine-disrupting chemicals (EDCs), benzo[α]pyrene (B[α]P) induced expression of CYP2N23, bisphenol A (BPA) induced CYP2P18 and CYP2P19, and 4-octylphenol (OP) induced CYP2AD12, but there was no significant response to 4-nonylphenol (NP), implying differential catalytic roles of the enzyme. In this paper, we identify and characterize a CYP2 gene cluster in the mangrove killifish K. marmoratus with differing catalytic roles toward EDCs. Our findings provide insights on the roles of nine co-localized CYP2 genes and their catalytic functions for better understanding of chemical-biological interactions in fish. © 2017 Elsevier B.V.
ISSN
0166-445X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1319
DOI
10.1016/j.aquatox.2017.03.022
Bibliographic Citation
Aquatic Toxicology, v.187, pp.132 - 140, 2017
Publisher
Elsevier B.V.
Subject
4 nonylphenol; 4 octylphenol; 4,4' isopropylidenediphenol; benzo[a]pyrene; cytochrome P450; endocrine disruptor; xenobiotic agent; 4,4' isopropylidenediphenol; 4-nonylphenol; 4-octylphenol; benzhydryl derivative; cytochrome P450; endocrine disruptor; phenol derivative; water pollutant; cyprinid; ecotoxicology; endocrine disruptor; gene expression; genetic analysis; genome; physiology; adult; animal experiment; animal tissue; Article; catalysis; controlled study; CYP2AD gene; CYP2N gene; CYP2P gene; embryo; embryo development; gene; gene cluster; gene duplication; gene expression; gene identification; gene location; genome; killifish; Kryptolebias marmoratus; male; nonhuman; phylogeny; priority journal; synteny; tissue specificity; zebra fish; animal; drug effects; gene expression; genetics; genome-wide association study; killifish; metabolism; multigene family; species difference; toxicity; water pollutant; Cyprinodontoidei; Danio rerio; Kryptolebias marmoratus; Oryzias latipes; Teleostei; Vertebrata; Animals; Benzhydryl Compounds; Cytochrome P-450 Enzyme System; Endocrine Disruptors; Gene Expression; Genome-Wide Association Study; Killifishes; Multigene Family; Phenols; Phylogeny; Species Specificity; Synteny; Water Pollutants, Chemical
Keywords
Cytochrome P450; Endocrine disrupting chemical; Gene cluster; Killifish; Kryptolebias marmoratus
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier B.V.
Related Researcher
Research Interests

Toxicology,Fish physiology,Marine environment risk assessment,독성학,어류생리학,해양환경 위해성평가

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