Genome-wide primary transcriptome analysis of H-2-producing archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

DC Field Value Language
dc.contributor.author Cho, Suhyung -
dc.contributor.author Kim, Min-Sik -
dc.contributor.author Jeong, Yujin -
dc.contributor.author Lee, Bo-Rahm -
dc.contributor.author Lee, Jung-Hyun -
dc.contributor.author Kang, Sung Gyun -
dc.contributor.author Cho, Byung-Kwan -
dc.date.accessioned 2020-04-16T10:55:10Z -
dc.date.available 2020-04-16T10:55:10Z -
dc.date.created 2020-01-28 -
dc.date.issued 2017-02-20 -
dc.identifier.issn 2045-2322 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/1286 -
dc.description.abstract In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of the H2-producing archaeon Thermococcus onnurineus NA1. We identified 1,082 purine-rich transcription initiation sites along with well-conserved TATA box, A-rich B recognition element (BRE), and promoter proximal element (PPE) motif in promoter regions, a high pyrimidine nucleotide content (T/C) at the-1 position, and Shine-Dalgarno (SD) motifs (GGDGRD) in 5' untranslated regions (5' UTRs). Along with differential transcript levels, 117 leaderless genes and 86 non-coding RNAs (ncRNAs) were identified, representing diverse cellular functions and potential regulatory functions under the different growth conditions. Interestingly, we observed low GC content in ncRNAs for RNA-based regulation via unstructured forms or interaction with other cellular components. Further comparative analysis of T. onnurineus upstream regulatory sequences with those of closely related archaeal genomes demonstrated that transcription of orthologous genes are initiated by highly conserved promoter sequences, however their upstream sequences for transcriptional and translational regulation are largely diverse. These results provide the genetic information of T. onnurineus for its future application in metabolic engineering. -
dc.description.uri 1 -
dc.language English -
dc.publisher NATURE PUBLISHING GROUP -
dc.subject LEADERLESS MESSENGER-RNA -
dc.subject INITIATION-FACTOR 2 -
dc.subject TRANSLATION INITIATION -
dc.subject START SITES -
dc.subject ESCHERICHIA-COLI -
dc.subject NONCODING RNAS -
dc.subject SEQUENCE -
dc.subject SEQ -
dc.subject ARCHITECTURE -
dc.subject PROMOTER -
dc.title Genome-wide primary transcriptome analysis of H-2-producing archaeon Thermococcus onnurineus NA1 -
dc.type Article -
dc.citation.title SCIENTIFIC REPORTS -
dc.citation.volume 7 -
dc.contributor.alternativeName 이정현 -
dc.contributor.alternativeName 강성균 -
dc.identifier.bibliographicCitation SCIENTIFIC REPORTS, v.7 -
dc.identifier.doi 10.1038/srep43044 -
dc.identifier.scopusid 2-s2.0-85013489218 -
dc.identifier.wosid 000394334500001 -
dc.type.docType Article -
dc.description.journalClass 1 -
dc.subject.keywordPlus LEADERLESS MESSENGER-RNA -
dc.subject.keywordPlus INITIATION-FACTOR 2 -
dc.subject.keywordPlus TRANSLATION INITIATION -
dc.subject.keywordPlus START SITES -
dc.subject.keywordPlus ESCHERICHIA-COLI -
dc.subject.keywordPlus NONCODING RNAS -
dc.subject.keywordPlus SEQUENCE -
dc.subject.keywordPlus SEQ -
dc.subject.keywordPlus ARCHITECTURE -
dc.subject.keywordPlus PROMOTER -
dc.relation.journalWebOfScienceCategory Multidisciplinary Sciences -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.relation.journalResearchArea Science & Technology - Other Topics -
Appears in Collections:
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse