Genome-wide primary transcriptome analysis of H-2-producing archaeon Thermococcus onnurineus NA1 SCIE SCOPUS

Cited 12 time in WEB OF SCIENCE Cited 12 time in Scopus
Title
Genome-wide primary transcriptome analysis of H-2-producing archaeon Thermococcus onnurineus NA1
Author(s)
Cho, Suhyung; Kim, Min-Sik; Jeong, Yujin; Lee, Bo-Rahm; Lee, Jung-Hyun; Kang, Sung Gyun; Cho, Byung-Kwan
KIOST Author(s)
Lee, Jung Hyun(이정현)Kang, Sung Gyun(강성균)
Publication Year
2017-02-20
Abstract
In spite of their pivotal roles in transcriptional and post-transcriptional processes, the regulatory elements of archaeal genomes are not yet fully understood. Here, we determine the primary transcriptome of the H2-producing archaeon Thermococcus onnurineus NA1. We identified 1,082 purine-rich transcription initiation sites along with well-conserved TATA box, A-rich B recognition element (BRE), and promoter proximal element (PPE) motif in promoter regions, a high pyrimidine nucleotide content (T/C) at the-1 position, and Shine-Dalgarno (SD) motifs (GGDGRD) in 5' untranslated regions (5' UTRs). Along with differential transcript levels, 117 leaderless genes and 86 non-coding RNAs (ncRNAs) were identified, representing diverse cellular functions and potential regulatory functions under the different growth conditions. Interestingly, we observed low GC content in ncRNAs for RNA-based regulation via unstructured forms or interaction with other cellular components. Further comparative analysis of T. onnurineus upstream regulatory sequences with those of closely related archaeal genomes demonstrated that transcription of orthologous genes are initiated by highly conserved promoter sequences, however their upstream sequences for transcriptional and translational regulation are largely diverse. These results provide the genetic information of T. onnurineus for its future application in metabolic engineering.
ISSN
2045-2322
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1286
DOI
10.1038/srep43044
Bibliographic Citation
SCIENTIFIC REPORTS, v.7, 2017
Publisher
NATURE PUBLISHING GROUP
Subject
LEADERLESS MESSENGER-RNA; INITIATION-FACTOR 2; TRANSLATION INITIATION; START SITES; ESCHERICHIA-COLI; NONCODING RNAS; SEQUENCE; SEQ; ARCHITECTURE; PROMOTER
Type
Article
Language
English
Document Type
Article
Publisher
NATURE PUBLISHING GROUP
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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