Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus SCIE SCOPUS

Cited 18 time in WEB OF SCIENCE Cited 22 time in Scopus
Title
Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
Author(s)
Song, Jung Min; Hong, Seung Kon; An, Young Jun; Kang, Mee Hye; Hong, Kwon Ho; Lee, Youn-Ho; Cha, Sun-Shin
KIOST Author(s)
An, Young Jun(안영준)Lee, Youn Ho(이윤호)
Alternative Author(s)
안영준; 이윤호
Publication Year
2017-03-01
Abstract
The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-,beta-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 degrees C, and maintained a half-life of 4 h at 70 degrees C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 degrees C, CaCel was effective to digest the green algae (Viva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.
ISSN
0021-8561
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1272
DOI
10.1021/acs.jafc.6b05037
Bibliographic Citation
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.65, no.8, pp.1630 - 1640, 2017
Publisher
AMER CHEMICAL SOC
Subject
MELANOCARPUS-ALBOMYCES; FEEDING GUILDS; CELLULASE GENE; PURIFICATION; EXPRESSION; CLONING; ENDOGLUCANASE; EVOLUTION; FAMILY; SERVER
Keywords
cold-active cellulase; Cryptopygus antarcticus; endo-beta-1,4-glucanase; horizontal gene transfer; biochemical and structural features; biofuel production from seaweeds
Type
Article
Language
English
Document Type
Article
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