Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
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Title
- Genetic and Structural Characterization of a Thermo-Tolerant, Cold-Active, and Acidic Endo-beta-1,4-glucanase from Antarctic Springtail, Cryptopygus antarcticus
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Author(s)
- Song, Jung Min; Hong, Seung Kon; An, Young Jun; Kang, Mee Hye; Hong, Kwon Ho; Lee, Youn-Ho; Cha, Sun-Shin
- KIOST Author(s)
- An, Young Jun(안영준); Lee, Youn Ho(이윤호)
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Alternative Author(s)
- 안영준; 이윤호
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Publication Year
- 2017-03-01
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Abstract
- The CaCel gene from Antarctic springtail Cryptopygus antarcticus codes for a cellulase belonging to the glycosyl hydrolase family 45 (GHF45). Phylogenetic, biochemical, and structural analyses revealed that the CaCel gene product (CaCel) is closely related to fungal GHF45 endo-,beta-1,4-glucanases. The organization of five introns within the open reading frame of the CaCel gene indicates its endogenous origin in the genome of the species, which suggests the horizontal transfer of the gene from fungi to the springtail. CaCel exhibited optimal activity at pH 3.5, retained 80% of its activity at 0-10 degrees C, and maintained a half-life of 4 h at 70 degrees C. Based on the structural comparison between CaCel and a fungal homologue, we deduced the structural basis for the unusual characteristics of CaCel. Under acidic conditions at 50 degrees C, CaCel was effective to digest the green algae (Viva pertusa), suggesting that it could be exploited for biofuel production from seaweeds.
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ISSN
- 0021-8561
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/1272
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DOI
- 10.1021/acs.jafc.6b05037
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Bibliographic Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.65, no.8, pp.1630 - 1640, 2017
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Publisher
- AMER CHEMICAL SOC
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Subject
- MELANOCARPUS-ALBOMYCES; FEEDING GUILDS; CELLULASE GENE; PURIFICATION; EXPRESSION; CLONING; ENDOGLUCANASE; EVOLUTION; FAMILY; SERVER
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Keywords
- cold-active cellulase; Cryptopygus antarcticus; endo-beta-1,4-glucanase; horizontal gene transfer; biochemical and structural features; biofuel production from seaweeds
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Type
- Article
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Language
- English
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Document Type
- Article
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