A heptameric peptide purified from Spirulina sp gastrointestinal hydrolysate inhibits angiotensin I-converting enzymeand angiotensin II-induced vascular dysfunction in human endothelial cells SCIE SCOPUS

Cited 14 time in WEB OF SCIENCE Cited 14 time in Scopus
Title
A heptameric peptide purified from Spirulina sp gastrointestinal hydrolysate inhibits angiotensin I-converting enzymeand angiotensin II-induced vascular dysfunction in human endothelial cells
Author(s)
Heo, Seong-Yeong; Ko, Seok-Chun; Kim, Chang Su; Oh, Gun-Woo; Ryu, Bomi; Qian, Zhong-Ji; Kim, Geunhyung; Park, Won Sun; Choi, Il-Whan; Phan, The Tuong Vy; Heo, Soo-Jin; Kang, Do-Hyung; Yi, Myunggi; Jung, Won-Kyo
KIOST Author(s)
Heo, Soo Jin(허수진)Kang, Do Hyung(강도형)
Publication Year
2017-05
Abstract
In this study, a marine microalga Spirulina sp.derived protein was hydrolyzed using gastrointestinal enzymes to produce an angiotensin I (Ang I)-converting enzyme (ACE) inhibitory peptide. Following consecutive purification, the potent ACE inhibitory peptide was composed of 7 amino acids, Thr-Met-Glu-Pro-Gly-Lys-Pro (molecular weight, 759 Da). Analysis using the Lineweaver-Burk plot and molecular modeling suggested that the purified peptide acted as a mixed non-competitive inhibitor of ACE. The inhibitory effects of the peptide against the cellular production of vascular dysfunction- related factors induced by Ang II were also investigated. In human endothelial cells, the Ang II-induced production of nitric oxide and reactive oxygen species was inhibited, and the expression of inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1) was downregulated when the cells were cultured with the purified peptide. Moreover, the peptide blocked the activation of p38 mitogen-activated protein kinase. These results indicated that this Spirulina sp.-derived peptide warrants further investigation as a potential pharmacological inhibitor of ACE and vascular dysfunction.
ISSN
1107-3756
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/1249
DOI
10.3892/ijmm.2017.2941
Bibliographic Citation
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.39, no.5, pp.1072 - 1082, 2017
Publisher
SPANDIDOS PUBL LTD
Subject
SPONTANEOUSLY HYPERTENSIVE-RATS; NITRIC-OXIDE SYNTHASE; SMOOTH-MUSCLE-CELLS; NANNOCHLOROPSIS-OCULATA; PROTEIN HYDROLYSATE; EXPRESSION; DAMAGE; STRESS; PURIFICATION; HYPERTROPHY
Keywords
Spirulina sp.; angiotensin I-converting enzyme; angiotensin II; vascular dysfunction; mitogen-activated protein kinases
Type
Article
Language
English
Document Type
Article
Publisher
SPANDIDOS PUBL LTD
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