A heptameric peptide purified from Spirulina sp gastrointestinal hydrolysate inhibits angiotensin I-converting enzymeand angiotensin II-induced vascular dysfunction in human endothelial cells SCIE SCOPUS
DC Field | Value | Language |
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dc.contributor.author | Heo, Seong-Yeong | - |
dc.contributor.author | Ko, Seok-Chun | - |
dc.contributor.author | Kim, Chang Su | - |
dc.contributor.author | Oh, Gun-Woo | - |
dc.contributor.author | Ryu, Bomi | - |
dc.contributor.author | Qian, Zhong-Ji | - |
dc.contributor.author | Kim, Geunhyung | - |
dc.contributor.author | Park, Won Sun | - |
dc.contributor.author | Choi, Il-Whan | - |
dc.contributor.author | Phan, The Tuong Vy | - |
dc.contributor.author | Heo, Soo-Jin | - |
dc.contributor.author | Kang, Do-Hyung | - |
dc.contributor.author | Yi, Myunggi | - |
dc.contributor.author | Jung, Won-Kyo | - |
dc.date.accessioned | 2020-04-16T10:40:13Z | - |
dc.date.available | 2020-04-16T10:40:13Z | - |
dc.date.created | 2020-01-28 | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/1249 | - |
dc.description.abstract | In this study, a marine microalga Spirulina sp.derived protein was hydrolyzed using gastrointestinal enzymes to produce an angiotensin I (Ang I)-converting enzyme (ACE) inhibitory peptide. Following consecutive purification, the potent ACE inhibitory peptide was composed of 7 amino acids, Thr-Met-Glu-Pro-Gly-Lys-Pro (molecular weight, 759 Da). Analysis using the Lineweaver-Burk plot and molecular modeling suggested that the purified peptide acted as a mixed non-competitive inhibitor of ACE. The inhibitory effects of the peptide against the cellular production of vascular dysfunction- related factors induced by Ang II were also investigated. In human endothelial cells, the Ang II-induced production of nitric oxide and reactive oxygen species was inhibited, and the expression of inducible nitric oxide synthase (iNOS) and endothelin-1 (ET-1) was downregulated when the cells were cultured with the purified peptide. Moreover, the peptide blocked the activation of p38 mitogen-activated protein kinase. These results indicated that this Spirulina sp.-derived peptide warrants further investigation as a potential pharmacological inhibitor of ACE and vascular dysfunction. | - |
dc.description.uri | 1 | - |
dc.language | English | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.subject | SPONTANEOUSLY HYPERTENSIVE-RATS | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | SMOOTH-MUSCLE-CELLS | - |
dc.subject | NANNOCHLOROPSIS-OCULATA | - |
dc.subject | PROTEIN HYDROLYSATE | - |
dc.subject | EXPRESSION | - |
dc.subject | DAMAGE | - |
dc.subject | STRESS | - |
dc.subject | PURIFICATION | - |
dc.subject | HYPERTROPHY | - |
dc.title | A heptameric peptide purified from Spirulina sp gastrointestinal hydrolysate inhibits angiotensin I-converting enzymeand angiotensin II-induced vascular dysfunction in human endothelial cells | - |
dc.type | Article | - |
dc.citation.endPage | 1082 | - |
dc.citation.startPage | 1072 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.citation.volume | 39 | - |
dc.citation.number | 5 | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 강도형 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.39, no.5, pp.1072 - 1082 | - |
dc.identifier.doi | 10.3892/ijmm.2017.2941 | - |
dc.identifier.scopusid | 2-s2.0-85018741393 | - |
dc.identifier.wosid | 000401073100002 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | SPONTANEOUSLY HYPERTENSIVE-RATS | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | SMOOTH-MUSCLE-CELLS | - |
dc.subject.keywordPlus | NANNOCHLOROPSIS-OCULATA | - |
dc.subject.keywordPlus | PROTEIN HYDROLYSATE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | DAMAGE | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | HYPERTROPHY | - |
dc.subject.keywordAuthor | Spirulina sp. | - |
dc.subject.keywordAuthor | angiotensin I-converting enzyme | - |
dc.subject.keywordAuthor | angiotensin II | - |
dc.subject.keywordAuthor | vascular dysfunction | - |
dc.subject.keywordAuthor | mitogen-activated protein kinases | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |